Changes in the peripheral blood transcriptome associated with occupational benzene exposure identified by cross-comparison on two microarray platforms.
Changes in the peripheral blood transcriptome associated with occupational benzene exposure identified by cross-comparison on two microarray platforms.
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DOI：
10.1016/j.ygeno.2008.12.006
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发表时间：
2009-04
期刊：
影响因子：
4.4
通讯作者：
Rothman, Nathaniel
中科院分区：
文献类型：
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作者：
McHale, Cliona M.;Zhang, Luoping;Lan, Qing;Li, Guilan;Hubbard, Alan E.;Forrest, Matthew S.;Vermeulen, Roel;Chen, Jinsong;Shen, Min;Rappaport, Stephen M.;Yin, Songnian;Smith, Martyn T.;Rothman, Nathaniel
苯是人类白血病和淋巴瘤的一个确定原因，但潜在的分子途径仍然很大程度上不确定。我们使用了两个微阵列平台，以确定全球基因表达的变化与良好的特征职业苯暴露在外周血单核细胞（PBMC）的制鞋工人的人口。发现了2692个基因（Affytelum）和1828个基因（Illumina）的差异表达，一致性为50%（基于两个平台的平均倍数变化≥ 1.3），一致基因之间的表达比例相似。我们之前通过微阵列鉴定并通过实时PCR确认的四个基因（CXCL16，ZNF 331，JUN和PF 4）是目前研究中两个平台鉴定的前100个基因之一。基因本体论分析表明，过度表达的基因参与细胞凋亡的一致基因，而途径分析确定的途径与脂质代谢。双平台方法允许识别苯暴露个体的PBMC转录组的稳健变化。
Benzene is an established cause of leukemia, and possibly lymphoma, in humans, but the underlying molecular pathways remain largely undetermined. We used two microarray platforms to identify global gene expression changes associated with well-characterized occupational benzene exposure in the peripheral blood mononuclear cells (PBMC) of a population of shoefactory workers. Differential expression of 2692 genes (Affymetrix) and 1828 genes (Illumina) was found and the concordance was 50 % (based on an average fold-change ≥ 1.3 from the two platforms), with similar expression ratios among the concordant genes. Four genes (CXCL16, ZNF331, JUN and PF4), which we previously identified by microarray and confirmed by real-time PCR, were among the top 100 genes identified by both platforms in the current study. Gene Ontology analysis showed overrepresentation of genes involved in apoptosis among the concordant genes while pathway analysis identified pathways related to lipid metabolism. The two-platform approach allows for robust changes in the PBMC transcriptome of benzene-exposed individuals to be identified.
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影响因子：
4
作者：
Hess, J;Angel, P;Schorpp-Kistner, M
通讯作者：
Schorpp-Kistner, M
影响因子：
5.8
作者：
Herrero, J;Díaz-Uriarte, R;Dopazo, J
通讯作者：
Dopazo, J
影响因子：
64.5
作者：
LANG, RA;BISHOP, JM
通讯作者：
BISHOP, JM
影响因子：
46.9
作者：
Canales, Roger D.;Luo, Yuling;Goodsaid, Federico M.
通讯作者：
Goodsaid, Federico M.
通过微阵列分析在暴露于苯的工人中发现新型生物标志物。
影响因子：
10.4
作者：
通讯作者：
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