The use of MR-detectable reporter molecules and ions to evaluate diffusion in normal and ischemic brain.
The use of MR-detectable reporter molecules and ions to evaluate diffusion in normal and ischemic brain.
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DOI：
10.1002/nbm.1530
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发表时间：
2010-08
影响因子：
2.9
通讯作者：
Neil, Jeffrey J.
中科院分区：
文献类型：
--
作者：
Ackerman, Joseph J. H.;Neil, Jeffrey J.
由于与区分由细胞内和细胞外水产生的MR信号相关的技术挑战，各种内源性和外源性MR可检测的分子和离子已被用作水运动的隔室特异性报告物。尽管这些报告分子不具有与水相同的表观扩散系数（ADC），但假定它们的ADC与它们在其中溶剂化的水的ADC直接相关。这种方法已被用于探测培养细胞和完整组织的细胞内和细胞外空间中的运动。尽管使用报告分子或离子存在潜在的解释性挑战，并且使用的种类繁多，但考虑到所有研究，以下结论是一致的：（i）细胞内空间中的表观自由扩散运动约为稀释水溶液中的一半;（ii）细胞内和细胞外水的ADC相似;（iii）细胞内ADC降低与脑损伤相关。这些发现为以下假设提供了支持：伴随脑损伤的总体脑水ADC降低主要是由细胞内水ADC降低驱动的。我们回顾了支持这些结论的研究，并在解释伴随脑损伤的整体脑水ADC下降的背景下对其进行解释。
As a result of the technical challenges associated with distinguishing the MR signals arising from intracellular and extracellular water, a variety of endogenous and exogenous MR-detectable molecules and ions have been employed as compartment-specific reporters of water motion. Although these reporter molecules do not have the same apparent diffusion coefficients (ADCs) as water, their ADCs are assumed to be directly related to the ADC of the water in which they are solvated. This approach has been used to probe motion in the intra- and extracellular space of cultured cells and intact tissue. Despite potential interpretative challenges with the use of reporter molecules or ions and the wide variety used, the following conclusions are consistent considering all studies: (i) the apparent free diffusive motion in the intracellular space is approximately one-half of that in dilute aqueous solution; (ii) ADCs for intracellular and extracellular water are similar; (iii) the intracellular ADC decreases in association with brain injury. These findings provide support for the hypothesis that the overall brain water ADC decrease that accompanies brain injury is driven primarily by a decrease in the ADC of intracellular water. We review the studies supporting these conclusions, and interpret them in the context of explaining the decrease in overall brain water ADC that accompanies brain injury.
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影响因子：
3.3
作者：
Komlosh, M. E.;Lizak, M. J.;Basser, P. J.
通讯作者：
Basser, P. J.
DOI：
10.1152/ajpcell.1996.271.6.c1895
发表时间：
1996-12-01
影响因子：
5.5
作者：
Hsu, EW;Aiken, NR;Blackband, SJ
通讯作者：
Blackband, SJ
影响因子：
--
作者：
HERBST, MD;GOLDSTEIN, JH
通讯作者：
GOLDSTEIN, JH
影响因子：
3.3
作者：
Goodman, JA;Kroenke, CD;Neil, JJ
通讯作者：
Neil, JJ
影响因子：
3.3
作者：
Anderson, AW;Zhong, JH;Gore, JC
通讯作者：
Gore, JC