Polyene-Producing Streptomyces spp. From the Fungus-Growing Termite Macrotermes barneyi Exhibit High Inhibitory Activity Against the Antagonistic Fungus Xylaria.
Polyene-Producing Streptomyces spp. From the Fungus-Growing Termite Macrotermes barneyi Exhibit High Inhibitory Activity Against the Antagonistic Fungus Xylaria.
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DOI：
10.3389/fmicb.2021.649962
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发表时间：
2021
影响因子：
5.2
通讯作者：
Ni J
中科院分区：
文献类型：
--
作者：
Li J;Sang M;Jiang Y;Wei J;Shen Y;Huang Q;Li Y;Ni J
真菌生长的白蚁从事与肠道微生物和单作（白蚁属）的三方互惠共生。在真菌花园里。白蚁常受昆虫病原菌（金龟子绿僵菌）困扰，真菌园常受到竞争对手（炭角菌属）的威胁。本研究旨在了解大白蚁肠道微生物的防御作用。我们获得了44个抗真菌菌株，表现出中度至强烈的抑制Xylaria sp. HPLC分析表明，不同类型的多烯（四烯，戊烯，和七烯）存在于10个强抗真菌链霉菌菌株的代谢产物。首次从链霉菌HF 10菌株中分离纯化了两种戊烯大环内酯类化合物（戊霉素和1′14-二羟基异链菌素），这两种化合物对炭角菌和木霉都具有较强的抑菌活性。绿僵菌比栽培种白蚁。随后的四烯和七烯相关基因破坏试验表明，突变株丧失了产生相应多烯的能力，对炭角菌和M.与野生型菌株相比，这些结果表明，产多烯链霉菌从M。barneyi对竞争菌有较强的抑制作用，而多烯对炭角菌有较强的抑制作用。
Fungus-growing termites are engaged in a tripartite mutualism with intestinal microbes and a monocultivar (Termitomyces sp.) in the fungus garden. The termites are often plagued by entomopathogen (Metarhizium anisopliae) and fungus garden is always threatened by competitors (Xylaria spp.). Here, we aim to understand the defensive role of intestinal microbes, the actinomycetes which were isolated from the gut of Macrotermes barneyi. We obtained 44 antifungal isolates, which showed moderate to strong inhibition to Xylaria sp. HPLC analysis indicated that different types of polyenes (tetraene, pentene, and heptaene) existed in the metabolites of 10 strong antifungal Streptomyces strains. Two pentene macrolides (pentamycin and 1′14-dihydroxyisochainin) were firstly purified from Streptomyces strain HF10, both exhibiting higher activity against Xylaria sp. and M. anisopliae than cultivar Termitomyces. Subsequently, tetraene and heptaene related gene disruption assay showed that the mutant strains lost the ability to produce corresponding polyenes, and they also had significantly decreased activities against Xylaria sp. and M. anisopliae compared to that of wild type strains. These results indicate that polyene-producing Streptomyces from the guts of M. barneyi have strong inhibition to competitor fungus and polyenes contribute to inhibitory effects on Xylaria sp.
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DOI：
10.1099/ijsem.0.004403
发表时间：
2020-10
影响因子：
2.8
作者：
Benndorf R;Martin K;Küfner M;de Beer ZW;Vollmers J;Kaster AK;Beemelmanns C
通讯作者：
Beemelmanns C
影响因子：
5.4
作者：
Bredholt, Harald;Fjaervik, Espen;Zotchev, Sergey B.
通讯作者：
Zotchev, Sergey B.
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
2.5
作者：
Chew, Yue Ming;Lye, SiewFen;Yahya, Adibah
通讯作者：
Yahya, Adibah
影响因子：
64.8
作者：
Currie, CR;Scott, JA;Malloch, D
通讯作者：
Malloch, D