A combined classical genetic and high resolution two-dimensional electrophoretic approach to the assessment of the number of genes affecting hybrid male sterility in Drosophila simulans and Drosophila sechellia.
A combined classical genetic and high resolution two-dimensional electrophoretic approach to the assessment of the number of genes affecting hybrid male sterility in Drosophila simulans and Drosophila sechellia.
复制标题
结合经典遗传和高分辨率二维电泳方法来评估影响果蝇和果蝇杂交雄性不育的基因数量。
作者：
Lingwen Zeng;Rama S. Singh
我们试图估计的基因数量在两个密切相关的物种，果蝇simulans和果蝇sechellia，通过一种新的方法，涉及使用高分辨率的二维凝胶电泳（2DE）检查父母，杂种和可育和不育回交后代的睾丸蛋白质。本研究的重要结果如下：（1）在1000种蛋白质中，约有8%的蛋白质表现出差异（存在/不存在）两个物种之间;（2）通过追踪亲本、杂种和回交雄性中的单个蛋白质，我们能够将不同的蛋白质与不同的染色体联系起来，并发现大多数不同的蛋白质与常染色体有关，很少与X染色体有关。（3）在杂种F_1雄虫中，无论在数量上还是在质量上都存在差异的蛋白质，绝大多数（97.4%）的蛋白质表达水平介于双亲之间，而在斑点密度上没有明显的变化。在两个亲本种中观察到的蛋白质，除两个可能由性染色体编码（或调控）的种特异性蛋白质外，其余蛋白质在F1杂种雄性中均存在。鉴定出塞切利亚特异性蛋白质与雄性不育性一致相关。这些结果沿着大部分回交一代的雄性可育性（23.6%），表明D. simulans和D. sechellia涉及相对少量的基因。不支持由于一般基因组不相容性引起的大规模遗传变化的作用。结果还表明，X染色体在杂种雄性不育中的巨大作用并不是因为X染色体比常染色体具有更高的分歧度。
We have attempted to estimate the number of genes involved in postzygotic reproductive isolation between two closely related species, Drosophila simulans and Drosophila sechellia, by a novel approach that involves the use of high resolution two-dimensional gel electrophoresis (2DE) to examine testis proteins in parents, hybrids and fertile and sterile backcross progenies. The important results that have emerged from this study are as follows: (1) about 8% of about 1000 proteins examined showed divergence (presence/absence) between the two species; (2) by tracing individual proteins in parental, hybrid and backcross males, we were able to associate the divergent proteins with different chromosomes and found that most divergent proteins are associated with autosomes and very few with X chromosome, Y chromosome and cytoplasm; (3) when proteins showing both quantitative and qualitative differences between the two species were examined in F1 hybrid males, most (97.4%) proteins were expressed at levels between the two parents and no sign of large scale changes in spot density was observed. All the proteins observed in the two parental species were present in F1 hybrid males except two species-specific proteins that may be encoded (or regulated) by sex chromosomes; (4) when different fertile and sterile backcross male testes were compared, a few D. sechellia-specific proteins were identified to be consistently associated with male sterility. These results along with the observation that a large proportion (23.6%) of first generation backcross males were fertile show that hybrid male sterility between D. simulans and D. sechellia involves a relatively small number of genes. Role of large scale genetic changes due to general genome incompatibility is not supported. The results also suggest that the large effect of X chromosome on hybrid male sterility is not due to higher divergence of X chromosome than autosomes.
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DOI：
--
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