Microporous nanofibrous fibrin-based scaffolds for bone tissue engineering.
Microporous nanofibrous fibrin-based scaffolds for bone tissue engineering.
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DOI：
10.1016/j.biomaterials.2008.06.030
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发表时间：
2008-10
期刊：
影响因子：
14
通讯作者：
Giachelli, Cecilia M.
中科院分区：
文献类型：
--
作者：
Osathanon, Thanaphum;Linnes, Michael L.;Rajachar, Rupak M.;Ratner, Buddy D.;Somerman, Martha J.;Giachelli, Cecilia M.
人体对合成聚合物的纤维化反应限制了它们在组织工程和其他应用中的成功。尽管多孔聚合物显示了更好的愈合能力，但控制其孔大小和孔互连的困难使人们对这一现象的理解变得模糊。在本研究中，开发了一种新型的制备天然聚合物/磷酸钙复合支架的方法，该支架具有严格可控的孔径、孔的连通性和钙磷沉积。采用球体模板法制备了微孔纳米纤维蛋白支架。通过将磷酸钙溶液沉积在纤维蛋白表面或直接掺入纳米晶羟基磷灰石(NHA)来制备复合支架。扫描电子显微镜结果表明，纤维蛋白支架具有高度多孔和相互连通的结构。从小鼠颅骨获得的成骨细胞样细胞在生物材料表面附着、铺展并呈多边形形态。可见多层细胞层和纤维基质沉积。此外，种植在矿化纤维蛋白支架上的细胞显示出明显高于纤维蛋白支架和nHA复合纤维蛋白支架(0.25g和0.5g)的碱性磷酸酶活性和成骨细胞标志基因的表达。所有类型的支架在体外和体内都被降解。此外，在小鼠颅骨缺损模型中，这些支架促进了骨形成，而添加重组人骨形态发生蛋白-2可以促进骨形成。
The fibrotic response of the body to synthetic polymers limits their success in tissue engineering and other applications. Though porous polymers have demonstrated improved healing, difficulty in controlling their pore sizes and pore interconnections has clouded the understanding of this phenomenon. In this study, a novel method to fabricate natural polymer/calcium phosphate composite scaffolds with tightly controllable pore size, pore interconnection, and calcium phosphate deposition was developed. Microporous, nanofibrous fibrin scaffolds were fabricated using sphere-templating methods. Composite scaffolds were created by solution deposition of calcium phosphate on fibrin surfaces or by direct incorporation of nanocrystalline hydroxyapatite (nHA). The SEM results showed that fibrin scaffolds exhibited a highly porous and interconnected structure. Osteoblast-like cells, obtained from murine calvaria, attached, spread and showed a polygonal morphology on the surface of the biomaterial. Multiple cell layers and fibrillar matrix deposition were observed. Moreover, cells seeded on mineralized fibrin scaffolds exhibited significantly higher alkaline phosphatase activity as well as osteoblast marker gene expression compared to fibrin scaffolds and nHA incorporated fibrin scaffolds (0.25 g and 0.5 g). All types of scaffolds were degraded both in vitro and in vivo. Furthermore, these scaffolds promoted bone formation in a mouse calvarial defect model and the bone formation was enhanced by addition of rhBMP-2.
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影响因子：
4.2
作者：
CHENG, PT
通讯作者：
CHENG, PT
影响因子：
4.1
作者：
Crane, Nicole J.;Popescu, Victoria;Ignelzi, Michael A., Jr.
通讯作者：
Ignelzi, Michael A., Jr.
DOI：
10.1002/jbm.b.30713
发表时间：
2007-07-01
影响因子：
3.4
作者：
Cronin, Kevin J.;Messina, Aurora;Knight, Kenneth R.
通讯作者：
Knight, Kenneth R.
影响因子：
14
作者：
Kim, SS;Park, MS;Kim, BS
通讯作者：
Kim, BS
影响因子：
14
作者：
Lee, YJ;Ko, JS;Kim, HM
通讯作者：
Kim, HM