Rifaximin Ameliorates Loperamide-Induced Constipation in Rats through the Regulation of Gut Microbiota and Serum Metabolites.
Rifaximin Ameliorates Loperamide-Induced Constipation in Rats through the Regulation of Gut Microbiota and Serum Metabolites.
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DOI：
10.3390/nu15214502
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发表时间：
2023-10-24
期刊：
影响因子：
5.9
通讯作者：
Xiong L
中科院分区：
文献类型：
--
作者：
Luo M;Xie P;Deng X;Fan J;Xiong L
肠道菌群的结构变化与功能性便秘的发生密切相关，调节肠道菌群可以改善便秘。利福昔明是一种吸收不良的抗生素，有利于调节肠道微生物群，但很少有研究报道其对便秘的影响。本研究的目的是研究利福昔明对洛哌丁胺诱导的SD大鼠便秘的影响。结果表明，利福昔明通过升高血清5-HT、SP、AQP 3、AQP 8的mRNA表达，降低TLR 2、TLR 4的mRNA表达而改善便秘。此外，利福昔明还能调节便秘大鼠肠道菌群，如增加潜在有益菌嗜粘蛋白阿克曼氏菌和鼠乳杆菌，减少假长双歧杆菌。代谢组学分析表明，便秘大鼠血清中胆汁酸和类固醇等代谢物发生变化，利福昔明干预后这些代谢物恢复正常。结论：利福昔明可能通过增加血清兴奋性神经递质和神经肽，调节水代谢，促进肠道炎症，改善洛哌丁胺诱导的大鼠便秘。多组学分析结果表明，利福昔明对便秘大鼠肠道菌群和血清代谢产物具有有益的调节作用，这可能在缓解便秘方面发挥关键作用。这项研究表明，利福昔明可能是一个潜在的策略，治疗便秘。
Structural changes in the gut microbiota are closely related to the development of functional constipation, and regulating the gut microbiota can improve constipation. Rifaximin is a poorly absorbed antibiotic beneficial for regulating gut microbiota, but few studies have reported its effects on constipation. The purpose of this study was to investigate the effect of rifaximin on loperamide-induced constipation in SD rats. The results showed that rifaximin improved constipation by increasing serum 5-HT, SP, and the mRNA expression of AQP3, AQP8, and reducing the mRNA expression of TLR2 and TLR4. In addition, rifaximin could regulate the gut microbiota of constipated rats, such as increasing the potentially beneficial bacteria Akkermansia muciniphila and Lactobacillus murinus, reducing the Bifidobacterium pseudolongum. According to metabolomics analysis, many serum metabolites, including bile acids and steroids, were changed in constipated rats and were recovered via rifaximin intervention. In conclusion, rifaximin might improve loperamide-induced constipation in rats by increasing serum excitatory neurotransmitters and neuropeptides, modulating water metabolism, and facilitating intestinal inflammation. Muti-Omics analysis results showed that rifaximin has beneficial regulatory effects on the gut microbiota and serum metabolites in constipated rats, which might play critical roles in alleviating constipation. This study suggests that rifaximin might be a potential strategy for treating constipation.
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影响因子：
6
作者：
通讯作者：
--
影响因子：
4.1
作者：
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3.5
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通讯作者：
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影响因子：
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