Lactobacillus plantarum KSFY06 and geniposide counteract montmorillonite-induced constipation in Kunming mice.
Lactobacillus plantarum KSFY06 and geniposide counteract montmorillonite-induced constipation in Kunming mice.
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DOI：
10.1002/fsn3.1814
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发表时间：
2020-09
影响因子：
3.9
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Gan Y;Liang J;Diao W;Zhou X;Mu J;Pang L;Tan F;Zhao X
便秘是消化系统疾病的常见临床表现，在世界范围内都有发生。本研究探讨植物乳杆菌KSFY06（LP-KSFY06）促进京尼平苷预防昆明小鼠蒙脱石所致便秘的作用，旨在提供成功的解决方案。测量了 LP-KSFY06 和京尼平苷对便秘的影响，结果表明 LP-KSFY06 增强了京尼平苷对便秘的保护作用，并且组合导致粪便重量、水分含量和颗粒数增加。第一次黑便排便时间从 182 分钟减少到 87 分钟，这清楚地表明便秘小鼠的排便困难得到缓解。 LP-KSFY06 和京尼平苷 (LP + G) 的协同干预有助于维持便秘小鼠的体重。 LP + G 干预显着增加了胃动素（MTL，167.8 pg/ml）、乙酰胆碱酯酶（AChE，45.3 pg/ml）、P 物质（SP，61.0 pg/ml）、血管活性肠肽（VIP，70.5 pg/ml）、内皮素-1（ET-1，16.1 pg/ml）和胃泌素的血清水平与 LP-KSFY06 组和京尼平苷组的这些指标相比，生长抑素（SS，35.2 pg/ml）和显着降低的生长抑素（SS，35.2 pg/ml）。 LP + G 治疗还显着增加分化簇 117 (c-Kit)、干细胞因子 (SCF)、胶质细胞源性神经营养因子 (GDNF) 的 mRNA 表达，并显着下调诱导型一氧化氮合酶 (iNOS)、瞬时受体电位香草酸-1 (TRPV1) 和环氧合酶-2 (COX-2) 的表达。实验结果表明，联合治疗对便秘的预防作用最强，LP-KSFY06促进京尼平苷预防便秘的能力。因此，LP-KSFY06是一种潜在的益生菌菌株，能够预防蒙脱石引起的便秘。植物乳杆菌KSFY06与京尼平苷联合作用可抑制蒙脱石所致昆明小鼠便秘。
Constipation is a common clinical manifestation of digestive system disorders and occurs worldwide. This study investigated the ability of Lactobacillus plantarum KSFY06 (LP‐KSFY06) to promote the action of geniposide in preventing montmorillonite‐induced constipation in Kunming mice, with the aim of providing a successful solution. The effects of LP‐KSFY06 and geniposide on constipation were measured, and the results showed that the protective effect of geniposide on constipation was enhanced by LP‐KSFY06 and that the combination resulted in increased weight, moisture content, and particle number of feces. The first black stool defecation time was decreased from 182 min to 87 min, which clearly indicates that defecating difficulty was alleviated in constipated mice. The synergic intervention of LP‐KSFY06 and geniposide (LP + G) assisted in maintaining the body weight of constipated mice. The LP + G intervention significantly increased serum levels of motilin (MTL, 167.8 pg/ml), acetylcholinesterase (AChE, 45.3 pg/ml), substance P (SP, 61.0 pg/ml), vasoactive intestinal peptide (VIP, 70.5 pg/ml), endothelin‐1 (ET‐1, 16.1 pg/ml), and gastrin (73.0 pg/ml) and remarkably decreased somatostatin (SS, 35.2 pg/ml) when compared to those indexes in the LP‐KSFY06 group and geniposide group. The LP + G treatment also significantly increased the mRNA expression of cluster of differentiation 117 (c‐Kit), stem cell factor (SCF), glial cell‐derived neurotrophic factor (GDNF), and remarkably downregulated the expression of inducible nitric oxide synthase (iNOS), transient receptor potential vanilloid‐1 (TRPV1), and cyclooxygenase‐2 (COX‐2). The experimental results showed that the combination treatment has the strongest prevention effect against constipation, and LP‐KSFY06 promotes the ability of geniposide to prevent constipation. Therefore, LP‐KSFY06 is a potential probiotic strain with the capacity to prevent montmorillonite‐induced constipation. Combined effects of Lactobacillus plantarum KSFY06 and geniposide could inhibit the montmorillonite‐induced constipation in Kunming mice.
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影响因子：
4.6
作者：
Nassif A;Longo WE;Sexe R;Stratton M;Standeven J;Vernava AM;Kaminski DL
通讯作者：
Kaminski DL
DOI：
10.1111/j.1440-1746.2005.03809.x
发表时间：
2005-08-01
影响因子：
4.1
作者：
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通讯作者：
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作者：
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通讯作者：
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DOI：
10.1016/j.ijbiomac.2019.04.209
发表时间：
2019-07-15
影响因子：
8.2
作者：
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通讯作者：
Cui, Hongli
影响因子：
2.7
作者：
Qian, Yu;Suo, Huayi;Liu, Zhenhu
通讯作者：
Liu, Zhenhu