The effect of scaffold macroporosity on angiogenesis and cell survival in tissue-engineered smooth muscle.
The effect of scaffold macroporosity on angiogenesis and cell survival in tissue-engineered smooth muscle.
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DOI：
10.1016/j.biomaterials.2014.03.025
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发表时间：
2014-06
期刊：
影响因子：
14
通讯作者：
Dunn, James C. Y.
中科院分区：
文献类型：
--
作者：
Walthers, Christopher M.;Nazemi, Alireza K.;Patel, Shilpy L.;Wu, Benjamin M.;Dunn, James C. Y.
血管生成和细胞在厚支架内的存活是组织工程中的主要关注点。本研究的目的是增加肠平滑肌细胞（SMCs）在植入的组织工程结构中的存活。我们将250 μm的孔纳入多层电纺支架中，大孔隙率范围为15%至25%，以促进血管生成。植入2周后评价表达绿色荧光蛋白（GFP）的SMC的存活率。而宿主细胞的浸润是类似的支架与不同的大孔隙率，血管的发展随着大孔隙率的增加而增加。具有25%大孔的支架具有最多的GFP表达SMC，这与距离最外层超过1mm的最高程度的血管生成相关。25%的大孔组超过了大孔连通性的临界阈值，加速了血管生成并提高了组织工程平滑肌构建体中植入细胞的存活率。
Angiogenesis and survival of cells within thick scaffolds is a major concern in tissue engineering. The purpose of this study is to increase the survival of intestinal smooth muscle cells (SMCs) in implanted tissue-engineered constructs. We incorporated 250-μm pores in multi-layered, electrospun scaffolds with a macroporosity ranging from 15% to 25% to facilitate angiogenesis. The survival of green fluorescent protein (GFP)-expressing SMCs was evaluated after 2 weeks of implantation. Whereas host cellular infiltration was similar in scaffolds with different macroporosities, blood vessel development increased with increasing macroporosity. Scaffolds with 25% macropores had the most GFP-expressing SMCs, which correlated with the highest degree of angiogenesis over 1 mm away from the outermost layer. The 25% macroporous group exceeded a critical threshold of macropore connectivity, accelerating angiogenesis and improving implanted cell survival in a tissue-engineered smooth muscle construct.
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