Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab(®).
Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab(®).
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DOI：
10.3390/jimaging5010017
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发表时间：
2019-01-14
影响因子：
3.2
通讯作者：
Reyes-Aldasoro CC
中科院分区：
文献类型：
--
作者：
Solís-Lemus JA;Stramer B;Slabaugh G;Reyes-Aldasoro CC
本文提出了一种新的软件框架，称为macrosight，它结合了例程来检测，跟踪和分析物体的形状和运动，特别强调巨噬细胞。macrosight中提出的关键特征包括一种算法，用于评估来自细胞-细胞接触的方向变化，其中假设发生相互作用。主要的生物学动机是确定影响细胞迁移的某些细胞相互作用。因此，这项工作的主要目标是提供见解的概念，细胞结构之间的相互作用导致方向的变化。Macrosight分析细胞在与另一个细胞接触之前和之后的方向变化。当细胞重叠并形成两个或多个细胞的团块时，确定相互作用。该框架将能够检测重叠细胞的分割技术和跟踪框架集成到用于分析细胞重叠之前和之后的轨迹的工具中。初步结果表明，承诺的分析和提出的假设，并奠定了基础，为进一步的发展。大量的实验和数据分析表明，在一定条件下，相互作用前后的运动变化与控制情况下的运动不同，具有统计学意义。
This paper presents a novel software framework, called macrosight, which incorporates routines to detect, track, and analyze the shape and movement of objects, with special emphasis on macrophages. The key feature presented in macrosight consists of an algorithm to assess the changes of direction derived from cell–cell contact, where an interaction is assumed to occur. The main biological motivation is the determination of certain cell interactions influencing cell migration. Thus, the main objective of this work is to provide insights into the notion that interactions between cell structures cause a change in orientation. Macrosight analyzes the change of direction of cells before and after they come in contact with another cell. Interactions are determined when the cells overlap and form clumps of two or more cells. The framework integrates a segmentation technique capable of detecting overlapping cells and a tracking framework into a tool for the analysis of the trajectories of cells before and after they overlap. Preliminary results show promise into the analysis and the hypothesis proposed, and lays the groundwork for further developments. The extensive experimentation and data analysis show, with statistical significance, that under certain conditions, the movement changes before and after an interaction are different from movement in controlled cases.
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DOI：
10.1083/jcb.200912134
发表时间：
2010-05-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Stramer B;Moreira S;Millard T;Evans I;Huang CY;Sabet O;Milner M;Dunn G;Martin P;Wood W
通讯作者：
Wood W
影响因子：
11.8
作者：
Wood W;Martin P
通讯作者：
Martin P
DOI：
10.1093/bioinformatics/btu080
发表时间：
2014-06-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Maška M;Ulman V;Svoboda D;Matula P;Matula P;Ederra C;Urbiola A;España T;Venkatesan S;Balak DM;Karas P;Bolcková T;Streitová M;Carthel C;Coraluppi S;Harder N;Rohr K;Magnusson KE;Jaldén J;Blau HM;Dzyubachyk O;Křížek P;Hagen GM;Pastor-Escuredo D;Jimenez-Carretero D;Ledesma-Carbayo MJ;Muñoz-Barrutia A;Meijering E;Kozubek M;Ortiz-de-Solorzano C
通讯作者：
Ortiz-de-Solorzano C
影响因子：
2
作者：
Reyes-Aldasoro, C. C.;Akerman, S.;Tozer, G. M.
通讯作者：
Tozer, G. M.
影响因子：
10.6
作者：
Zimmer, C;Labruyère, E;Olivo-Marin, JC
通讯作者：
Olivo-Marin, JC