Proteomic analysis reveals rotator cuff injury caused by oxidative stress.
Proteomic analysis reveals rotator cuff injury caused by oxidative stress.
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DOI：
10.1177/2040622320987057
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发表时间：
2021
影响因子：
3.5
通讯作者：
Bao NR
中科院分区：
文献类型：
--
作者：
Yuan T;Qian H;Yu X;Meng J;Lai CT;Jiang H;Zhao JN;Bao NR
肩袖肌腱病是一种常见病，与疼痛和功能障碍有关。然而，肩袖损伤和肩痛的病理机制尚不清楚。目的：探讨肩袖损伤和肩痛的病理机制，并利用蛋白质组学技术筛选与肩袖损伤相关的标志蛋白。肩峰下滑膜标本采集自接受肩关节镜手术的患者。实验组为肩袖修复术患者，对照组为习惯性肩关节脱位患者。进行病理学检查，然后进行非标记定量蛋白质组学检测。最后，通过对样本生物学信息的分析，对差异蛋白进行功能分析，推断出与肩袖损伤和肩痛相关的特异性蛋白。实验组患者均具有代表性。实验组病理切片可见大量脂肪细胞和炎性细胞;蛋白质组学分析筛选出80个差异显著的蛋白质，蛋白质功能分析表明，S100 A11（p = 0.011）、PLIN 4（p = 0.017）、HYOU 1（p = 0.002）和CLIC 1（p = 0.007）与氧化应激和慢性炎症密切相关。肩袖损伤与氧化应激和慢性炎症反应密切相关，S100 A11、PLIN 4、HYOU 1和CLIC 1在肩袖损伤滑膜中的表达为研究其病理机制提供了新的标志物。
Rotator cuff tendinopathy is common and is related to pain and dysfunction. However, the pathological mechanism of rotator cuff injury and shoulder pain is unclear. Objective: to investigate the pathological mechanism of rotator cuff injury and shoulder pain, and screen out the marker proteins related to rotator cuff injury by proteomics. Subacromial synovium specimens were collected from patients undergoing shoulder arthroscopic surgery. The experimental group were patients with rotator cuff repair surgery, and the control group were patients with habitual dislocation of the shoulder joint. Pathological examination was performed, and then followed by non-labeled quantitative proteomic detection. Finally, from analysis of the biological information of the samples, specific proteins related to rotator cuff injury and shoulder pain were deduced by functional analysis of differential proteins. All the patients in experimental groups were representative. A large number of adipocytes and inflammatory cells were found in the pathological sections of the experimental group; the proteomics analysis screen identified 80 proteins with significant differences, and the analysis of protein function revealed that S100A11 (p = 0.011), PLIN4 (p = 0.017), HYOU1 (p = 0.002) and CLIC1 (p = 0.007) were closely related to oxidative stress and chronic inflammation. Rotator cuff injury is closely related to oxidative stress and chronic inflammatory response, and the results suggest that the expression of S100A11, PLIN4, HYOU1 and CLIC1 in the synovium of rotator cuff injury provides a new marker for the study of its pathological mechanism.
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DOI：
10.26355/eurrev_201901_17914
发表时间：
2019-05-01
影响因子：
3.3
作者：
Li, X.;Zhang, N-X;Wang, N-N
通讯作者：
Wang, N-N
影响因子：
3.8
作者：
Jo, Chris Hyunchul;Shin, Won Hyoung;Kim, Ji Eun
通讯作者：
Kim, Ji Eun
DOI：
10.1002/jor.23662
发表时间：
2018-03
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Gibbons MC;Singh A;Engler AJ;Ward SR
通讯作者：
Ward SR
影响因子：
5
作者：
Li, Meng;van Esch, Betty C. A. M.;Henricks, Paul A. J.
通讯作者：
Henricks, Paul A. J.
影响因子：
6.9
作者：
Choromanska, Barbara;Mysliwiec, Piotr;Miklosz, Agnieszka
通讯作者：
Miklosz, Agnieszka