Genomic and proteomic comparisons of bacteriocins in probiotic species Lactobacillus and Bifidobacterium and inhibitory ability of Escherichia coli MG 1655.
Genomic and proteomic comparisons of bacteriocins in probiotic species Lactobacillus and Bifidobacterium and inhibitory ability of Escherichia coli MG 1655.
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益生菌乳杆菌和双歧杆菌细菌素的基因组和蛋白质组学比较及大肠杆菌MG1655的抑制能力。
DOI：
10.1016/j.btre.2021.e00654
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Sadrnia M
中科院分区：
文献类型：
--
作者：
Darvishi N;Fard NA;Sadrnia M
对12种双歧杆菌和46种乳酸菌的基因组和蛋白质组进行了分析，并进行了比较。NCBI-Genome、UniProt-Proteome、Bactibase和BAGL4数据库以及BLASTP和Clustal Omega可用于细菌素的挖掘。与双歧杆菌相比，乳杆菌种类的细菌素具有更大的多样性和丰度。其中，酒糟乳杆菌、平板乳杆菌、罗伊氏乳杆菌、发酵乳杆菌和干酪乳杆菌对病原菌的抑制作用最强（大肠杆菌MG 1655）；分别。一组乳酸菌包括酒井乳杆菌、罗伊氏乳杆菌、发酵乳杆菌和干酪乳杆菌，可以作为一种生物安全的解决方案来控制胃肠道病原体。细菌素是一大类细菌多肽或蛋白质，由核糖体合成，对其他细菌具有抗菌活性。我们使用NCBI-Genome、UniProt-Proteome、Bactibase和BAGL4数据库对12种双歧杆菌和46种乳酸菌的细菌素基因组和蛋白质组进行了研究和比较。对所选乳酸菌进行胆盐耐药、酸和pH耐药、胃蛋白酶和胰蛋白酶酶耐药和抗生素耐药检测。同时，对所选乳酸菌对大肠杆菌MG 1655的抑菌活性进行了评价。结果表明，乳杆菌比双歧杆菌具有更丰富的细菌素多样性和丰度。其中，酒糟乳杆菌、平板乳杆菌、罗伊氏乳杆菌、发酵乳杆菌和干酪乳杆菌的抑菌效果最好；分别。因此，这些乳酸菌的组合可以作为控制胃肠道病原体的生化和安全解决方案，以及食品技术中抗生素和化学品的合适替代品。
The genomes and proteomes of 12 Bifidobacterium and 46 Lactobacillus were reviewed and then compared for bacteriocin identification. NCBI-Genome, UniProt-Proteome, Bactibase, and BAGL4 databases, as well as BLASTP, and Clustal Omega can be used for bacteriocin mining. Lactobacillus species have more diversity and abundance of bacteriocin compared to Bifidobacterium species. Notably, L. sakei, L. plamtarum, L. reuteri, L. fermentum, and L. casei had the highest pathogen inhibition (E. coli MG 1655); respectively. A set of Lactobacillus bacteria including L. sakei, L. reuteri, L. fermentum, and L. casei can be proposed as a biosecure and safe solution to control gastrointestinal pathogens. Bacteriocins are a large family of bacterial peptides or proteins, ribosomally synthesized with antimicrobial activity against other bacteria. We investigated and compared the genomes and proteomes of 12 Bifidobacterium and 46 Lactobacillus species for bacteriocins using NCBI-Genome, UniProt-Proteome, Bactibase, and BAGL4 databases. Selected Lactobacillus species were examined for bile salt resistance, acid and pH resistance, pepsin and trypsin enzyme resistance, and antibiotic resistance. Also, the antimicrobial activity of selected Lactobacillus species was evaluated against E. coli MG 1655. Results showed that Lactobacillus species have more diversity and abundance of bacteriocin compared to Bifidobacterium species. Notably, L. sakei, L. plamtarum, L. reuteri, L. fermentum, and L. casei had the highest pathogen inhibition; respectively. Therefore, a combination of these Lactobacillus species can be suggested as a biochemical and safe solution to control gastrointestinal pathogens and suitable alternatives to antibiotics and chemicals in food technology.
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DOI：
10.1016/s0002-8223(01)00060-8
发表时间：
2001-02-01
影响因子：
--
作者：
Kopp-Hoolihan, L
通讯作者：
Kopp-Hoolihan, L
影响因子：
3.5
作者：
Gomes, AMP;Malcata, FX;Klaver, FAM
通讯作者：
Klaver, FAM
影响因子：
4.9
作者：
Morgan, SM;O'Connor, PM;Hill, C
通讯作者：
Hill, C
影响因子：
4.6
作者：
Chopra L;Singh G;Kumar Jena K;Sahoo DK
通讯作者：
Sahoo DK
影响因子：
3.2
作者：
Gründling, A;Schneewind, O
通讯作者：
Schneewind, O