The genome of oil-Camellia and population genomics analysis provide insights into seed oil domestication.
The genome of oil-Camellia and population genomics analysis provide insights into seed oil domestication.
复制标题
油茶花基因组和群体基因组学分析为种子油驯化提供了见解
DOI：
10.1186/s13059-021-02599-2
复制
发表时间：
2022-01-10
期刊：
影响因子：
12.3
通讯作者：
Yin H
中科院分区：
文献类型：
--
作者：
Lin P;Wang K;Wang Y;Hu Z;Yan C;Huang H;Ma X;Cao Y;Long W;Liu W;Li X;Fan Z;Li J;Ye N;Ren H;Yao X;Yin H
油茶是一种多年生作物，具有悠久的驯化历史，生产的油茶籽油品质优良，对人体健康有益。油茶(Camellia oleifera Abel.是茶树的姊妹种，茶树被广泛种植用于食用油生产。然而，由于缺乏足够的基因组信息，油茶驯化的分子机制研究仍然受到限制。为了阐明进化和驯化的遗传和基因组学基础，本文报道了野生油茶染色体尺度的参考基因组（2.95Gb），以及221个栽培品种的转录组测序数据。油茶基因组由多种测序技术整合而成，重复序列比例高（76.1%），杂合性高（2.52%）。通过对控制授粉杂交后代进行测序，构建了高密度校正标记遗传图谱。全基因组关联研究揭示了参与油脂生物合成和植物激素途径的人工选择基因的子集。特别是，我们发现了糖依赖性三酰甘油脂肪酶1、β-酮酰基-酰基载体蛋白合成酶III和硬脂酰基-酰基载体蛋白去饱和酶基因的优良等位基因，这些等位基因在油茶驯化过程中对提高种子油的产量和品质具有重要作用。本研究构建了油茶植物染色体尺度的参考基因组，并证明了人工选择与油脂生物合成相关基因的优良等位基因有助于油茶的驯化。在线版本包含补充材料，可登录10.1186/s13059-021-02599-2获取。
As a perennial crop, oil-Camellia possesses a long domestication history and produces high-quality seed oil that is beneficial to human health. Camellia oleifera Abel. is a sister species to the tea plant, which is extensively cultivated for edible oil production. However, the molecular mechanism of the domestication of oil-Camellia is still limited due to the lack of sufficient genomic information. To elucidate the genetic and genomic basis of evolution and domestication, here we report a chromosome-scale reference genome of wild oil-Camellia (2.95 Gb), together with transcriptome sequencing data of 221 cultivars. The oil-Camellia genome, assembled by an integrative approach of multiple sequencing technologies, consists of a large proportion of repetitive elements (76.1%) and high heterozygosity (2.52%). We construct a genetic map of high-density corrected markers by sequencing the controlled-pollination hybrids. Genome-wide association studies reveal a subset of artificially selected genes that are involved in the oil biosynthesis and phytohormone pathways. Particularly, we identify the elite alleles of genes encoding sugar-dependent triacylglycerol lipase 1, β-ketoacyl-acyl carrier protein synthase III, and stearoyl-acyl carrier protein desaturases; these alleles play important roles in enhancing the yield and quality of seed oil during oil-Camellia domestication. We generate a chromosome-scale reference genome for oil-Camellia plants and demonstrate that the artificial selection of elite alleles of genes involved in oil biosynthesis contributes to oil-Camellia domestication. The online version contains supplementary material available at 10.1186/s13059-021-02599-2.
登录
查看更多内容
影响因子：
9.2
作者：
Chang CC;Chow CC;Tellier LC;Vattikuti S;Purcell SM;Lee JJ
通讯作者：
Lee JJ
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
3.8
作者：
O'Neill, C. M.;Morgan, C.;Hattori, C.;Brennan, M.;Rosas, U.;Tschoep, H.;Deng, P. X.;Baker, D.;Wells, R.;Bancroft, I.
通讯作者：
Bancroft, I.
影响因子：
13.8
作者：
Kanai, Masatake;Mano, Shoji;Nishimura, Mikio
通讯作者：
Nishimura, Mikio
玉米驯化和改进的比较人口基因组学。
影响因子：
30.8
作者：
Hufford MB;Xu X;van Heerwaarden J;Pyhäjärvi T;Chia JM;Cartwright RA;Elshire RJ;Glaubitz JC;Guill KE;Kaeppler SM;Lai J;Morrell PL;Shannon LM;Song C;Springer NM;Swanson-Wagner RA;Tiffin P;Wang J;Zhang G;Doebley J;McMullen MD;Ware D;Buckler ES;Yang S;Ross-Ibarra J
通讯作者：
Ross-Ibarra J