Interstitial cell network volume is reduced in the terminal bowel of ageing mice.
Interstitial cell network volume is reduced in the terminal bowel of ageing mice.
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DOI：
10.1111/jcmm.13794
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发表时间：
2018-10
影响因子：
5.3
通讯作者：
Saffrey MJ
中科院分区：
文献类型：
--
作者：
Gamage PPKM;Patel BA;Yeoman MS;Ranson RN;Saffrey MJ
衰老与末端胃肠道的神经肌肉功能受损有关，这可能导致慢性便秘、粪便嵌塞和尿失禁。cajal间质细胞（ICC）在调节肠平滑肌收缩中起重要作用。然而，终末胃肠道（肛管包括肛门括约肌区和直肠）ICC体积随年龄的变化尚未研究。本文研究了3 ~ 4月龄和26 ~ 28月龄C57BL/6小鼠末端胃肠道ICC的分布、形态和网络体积。用c‐Kit抗体对整批制剂进行免疫荧光标记，鉴定ICC。ICC网络体积通过共焦Z堆叠的基于软件的三维体绘制来测量。在老年动物中，每单位肌肉体积的ICC网络体积显著减少。未检测到ICC形态学与年龄相关的变化。肛门括约肌区和直肠圆形肌层厚度随年龄增长而增加，结肠远端圆形肌层厚度随年龄增长而减少。这些结果表明，衰老与末端胃肠道ICC网络体积的减少有关，这可能会影响这些区域的正常功能。
Ageing is associated with impaired neuromuscular function of the terminal gastrointestinal (GI) tract, which can result in chronic constipation, faecal impaction and incontinence. Interstitial cells of cajal (ICC) play an important role in regulation of intestinal smooth muscle contraction. However, changes in ICC volume with age in the terminal GI tract (the anal canal including the anal sphincter region and rectum) have not been studied. Here, the distribution, morphology and network volume of ICC in the terminal GI tract of 3‐ to 4‐month‐old and 26‐ to 28‐month‐old C57BL/6 mice were investigated. ICC were identified by immunofluorescence labelling of wholemount preparations with an antibody against c‐Kit. ICC network volume was measured by software‐based 3D volume rendering of confocal Z stacks. A significant reduction in ICC network volume per unit volume of muscle was measured in aged animals. No age‐associated change in ICC morphology was detected. The thickness of the circular muscle layer of the anal sphincter region and rectum increased with age, while that in the distal colon decreased. These results suggest that ageing is associated with a reduction in the network volume of ICC in the terminal GI tract, which may influence the normal function of these regions.
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影响因子：
--
作者：
Saffrey, M. Jill
通讯作者：
Saffrey, M. Jill
影响因子：
5.5
作者：
Hall, K. A.;Ward, S. M.;Keef, K. D.
通讯作者：
Keef, K. D.
影响因子：
3.3
作者：
Yu, Siegfried W. B.;Rao, Satish S. C.
通讯作者：
Rao, Satish S. C.
影响因子：
4.6
作者：
Patel BA;Fidalgo S;Wang C;Parmar L;Mandona K;Panossian A;Flint MS;Ranson RN;Saffrey MJ;Yeoman MS
通讯作者：
Yeoman MS
DOI：
10.1152/ajpgi.00304.2012
发表时间：
2014-04-01
影响因子：
4.5
作者：
Jo, Hyun Jin;Kim, Nayoung;Jung, Hyun Chae
通讯作者：
Jung, Hyun Chae