The 14;18 translocation in European cases of follicular lymphoma: comparison of Southern blotting and the polymerase chain reaction
The 14;18 translocation in European cases of follicular lymphoma: comparison of Southern blotting and the polymerase chain reaction
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欧洲滤泡性淋巴瘤病例中的 14;18 易位：Southern blotting 和聚合酶链反应的比较
DOI：
10.1111/j.1365-2141.1990.tb07836.x
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发表时间：
1990
影响因子：
6.5
通讯作者：
D. Mason
中科院分区：
文献类型：
--
作者：
F. Pezzella;E. Ralfkiaer;K. Gatter;D. Mason
总结。14;18染色体易位被广泛认为是一种与滤泡性淋巴瘤相关的细胞遗传学异常，但其在这类淋巴瘤中的发生率从不到50%到近90%不等。此外，欧洲滤泡性淋巴瘤病例中t（14;18）的频率没有广泛的数据发表。来自两个欧洲中心（牛津和哥本哈根）的51例滤泡性淋巴瘤患者的淋巴结检查了这种易位的存在。26例采用Southern印迹法和聚合酶链反应（PCR）， 25例仅采用PCR法（其中仅有降解DNA或福尔马林固定样品）。DNA探针能够检测重排在主要和次要的断点区域被采用。51例中只有21例（41%）可以检测到t（14:18）。然而，对文献的回顾显示，以前使用细胞遗传学和分子生物学技术获得了类似的结果，我们的结果支持t（14;18）在滤泡性淋巴瘤中的全球发病率不大于70%的观点。此外，本研究表明，当只有福尔马林固定石蜡包埋组织或降解DNA可用时，PCR是鉴定t（14:18）的可靠方法。
Summary. The 14;18 chromosomal translocation is widely recognized as a cytogenetic abnormality associated with follicular lymphomas, but estimates of its frequency in this type of lymphoma vary widely from less than 50% to almost 90%. Furthermore, no extensive data have been published on the frequency of t(14;18) in European cases of follicular lymphoma. Lymph nodes from 51 patients with follicular lymphomas obtained from two European centres (Oxford and Copenhagen) were examined for the presence of this translocation. Southern blotting and the polymerase chain reaction (PCR) were used in 26 cases and the PCR alone in 25 cases (from which only degraded DNA or formalin fixed samples were available). DNA probes capable of detecting rearrangement at both the major and the minor breakpoint regions were employed. We could detect t(14:18) in only 21 out of 51 cases (41%). However, a review of the literature showed that comparable results have been obtained previously using both cytogenetic and molecular biological techniques and our results support the view that the global incidence of t(14;18) in follicular lymphoma is no greater than 70%. Furthermore, this study has indicated that the PCR is a reliable method for identifying t(14:18) when only formalin‐fixed paraffin‐embedded tissue or degraded DNA is available.
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影响因子：
56.9
作者：
TSUJIMOTO, Y;FINGER, LR;CROCE, CM
通讯作者：
CROCE, CM
影响因子：
8
作者：
Y. Tsujimoto;N. Ikegaki;C. Croce
通讯作者：
Y. Tsujimoto;N. Ikegaki;C. Croce
DOI：
10.1073/pnas.83.14.5214
发表时间：
1986-07-01
影响因子：
11.1
作者：
TSUJIMOTO, Y;CROCE, CM
通讯作者：
CROCE, CM
影响因子：
56.9
作者：
TSUJIMOTO, Y;COSSMAN, J;CROCE, CM
通讯作者：
CROCE, CM
DOI：
10.1073/pnas.82.21.7439
发表时间：
1985-01-01
影响因子：
11.1
作者：
CLEARY, ML;SKLAR, J
通讯作者：
SKLAR, J