The lipid transfer protein OsLTPL159 is involved in cold tolerance at the early seedling stage in rice
The lipid transfer protein OsLTPL159 is involved in cold tolerance at the early seedling stage in rice
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脂质转运蛋白OsLTPL159参与水稻苗期早期的耐寒性
DOI：
10.1111/pbi.13243
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发表时间：
2019-09
影响因子：
13.8
通讯作者：
Liu Fengxia
中科院分区：
文献类型：
--
作者：
Zhao Jie;Wang Shanshan;Qin Jingjing;Sun Chuanqing;Liu Fengxia
非特异性脂质转运蛋白（nsLTP）在植物发育和对非生物胁迫的响应中起着关键作用。在此，我们发现水稻脂质转运蛋白OsLTPL159与幼苗早期的耐冷性相关。在粳稻品种中花17（ZH17）或籼稻品种特青背景中过表达来自耐冷渐渗系IL112的OsLTPL159 IL112等位基因显著增强了耐冷性。此外，通过RNA干扰（RNAi）下调粳稻ZH17中OsLTPL159的表达显著降低了耐冷性。进一步的转录组学、生理学和组织学分析表明，OsLTPL 159 IL 112等位基因可能通过降低活性氧的毒害作用，促进细胞壁纤维素沉积和渗透压物质积累，从而维持叶绿体的完整性，增强水稻幼苗早期的耐冷性。值得注意的是，来自受体亲本桂朝2号（GC 2）的另一个等位基因OsLTPL 159 GC 2的过表达没有提高耐冷性，表明GC 2的OsLTPL 159编码区的变异可能破坏了其耐冷性功能。进一步序列比较发现，所调查的22个粳稻品种均具有与IL112相同的OsLTPL159单倍型，并且比所调查的籼稻品种更耐冷，这意味着OsLTPL159的变异可能与粳稻和籼稻的耐冷性差异有关。因此，我们的研究结果表明，水稻OsLTPL159等位基因可以通过分子育种策略来提高籼稻的耐冷性。
Summary Nonspecific lipid transfer proteins (nsLTPs) play critical roles in plant development and response to abiotic stresses. Here, we found that a rice lipid transfer protein, OsLTPL159, was associated with cold tolerance at the early seedling stage. Overexpression of an OsLTPL159 IL 112 allele from the cold‐tolerant introgression line IL112 in either the japonica variety Zhonghua17 (ZH17) or the indica variety Teqing background dramatically enhanced cold tolerance. In addition, down‐regulation of the expression of OsLTPL159 in the japonica variety ZH17 by RNA interference (RNAi) significantly decreased cold tolerance. Further transcriptomic, physiological and histological analysis showed that the OsLTPL159 IL 112 allele likely enhanced the cold tolerance of rice at the early seedling stage by decreasing the toxic effect of reactive oxygen species, enhancing cellulose deposition in the cell wall and promoting osmolyte accumulation, thereby maintaining the integrity of the chloroplasts. Notably, overexpression of another allele, OsLTPL159 GC 2, from the recipient parent Guichao 2 (GC2), an indica variety, did not improve cold tolerance, indicating that the variations in the OsLTPL159 coding region of GC2 might disrupt its function for cold tolerance. Further sequence comparison found that all 22 japonica varieties surveyed had an OsLTPL159 haplotype identical to IL112 and were more cold‐tolerant than the surveyed indica varieties, implying that the variations in OsLTPL159 might be associated with differential cold tolerance of japonica and indica rice. Therefore, our findings suggest that the OsLTPL159 allele of japonica rice could be used to improve cold tolerance of indica rice through a molecular breeding strategy.
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agriGO v2.0：农业社区的 GO 分析工具包，2017 年更新
影响因子：
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