Non-invasive neuromodulation using rTMS and the electromagnetic-perceptive gene (EPG) facilitates plasticity after nerve injury.
Non-invasive neuromodulation using rTMS and the electromagnetic-perceptive gene (EPG) facilitates plasticity after nerve injury.
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DOI：
10.1016/j.brs.2020.10.006
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发表时间：
2020-11
影响因子：
7.7
通讯作者：
Pelled G
中科院分区：
文献类型：
--
作者：
Cywiak C;Ashbaugh RC;Metto AC;Udpa L;Qian C;Gilad AA;Reimers M;Zhong M;Pelled G
2000万美国人患有外周神经损伤。这些患者通常会出现慢性疼痛和感觉功能障碍。在过去的十年中，神经影像学研究表明，这些变化与改变皮层兴奋-抑制平衡和适应不良的可塑性。我们测试了剥夺感觉皮层的神经调节是否可以恢复皮层平衡，以及它是否能有效减轻感觉并发症。我们测试了诱导神经元兴奋性的非侵入性重复经颅磁刺激（rTMS）和通过电磁感知基因（EPG）的细胞特异性磁激活是否可以恢复皮质兴奋性，所述电磁感知基因是从玻璃鲶鱼中鉴定和克隆的新基因，并且被证明在磁刺激时引起神经反应。使用大鼠前爪去神经模型。从急性或慢性损伤后阶段开始，每隔一天给予rTMS，持续30天。从慢性期开始，每天通过EPG进行微创神经调节30次。在肢体失神经支配后的几天和几周内，在EPG治疗的大鼠中进行了一系列行为测试，并在rTMS治疗的大鼠中进行了行为测试，fMRI和免疫化学。结果表明，神经调节显著改善了长期活动性，减少了焦虑，增强了神经可塑性。结果表明，急性和延迟rTMS干预促进康复。此外，这些结果暗示EPG是一种有效的细胞特异性神经调节方法。总之，这些结果加强了来自人类和动物研究的越来越多的证据，这些研究将神经调节作为促进可塑性和康复的有效策略。
Twenty million Americans suffer from peripheral nerve injury. These patients often develop chronic pain and sensory dysfunctions. In the past decade, neuroimaging studies showed that these changes are associated with altered cortical excitation-inhibition balance and maladaptive plasticity. We tested if neuromodulation of the deprived sensory cortex could restore the cortical balance, and whether it would be effective in alleviating sensory complications. We tested if non-invasive repetitive transcranial magnetic stimulation (rTMS) which induces neuronal excitability, and cell-specific magnetic activation via the Electromagnetic-perceptive gene (EPG) which is a novel gene that was identified and cloned from glass catfish and demonstrated to evoke neural responses when magnetically stimulated, can restore cortical excitability. A rat model of forepaw denervation was used. rTMS was delivered every other day for 30 days, starting at the acute or at the chronic post-injury phase. A minimally-invasive neuromodulation via EPG was performed every day for 30 staring at the chronic phase. A battery of behavioral tests was performed in the days and weeks following limb denervation in EPG-treated rats, and behavioral tests, fMRI and immunochemistry were performed in rTMS-treated rats. The results demonstrate that neuromodulation significantly improved long-term mobility, decreased anxiety and enhanced neuroplasticity. The results identify that both acute and delayed rTMS intervention facilitated rehabilitation. Moreover, the results implicate EPG as an effective cell-specific neuromodulation approach. Together, these results reinforce the growing amount of evidence from human and animal studies that are establishing neuromodulation as an effective strategy to promote plasticity and rehabilitation.
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影响因子：
8.3
作者：
Celnik, Pablo;Paik, Nam-Jong;Cohen, Leonardo G.
通讯作者：
Cohen, Leonardo G.
影响因子：
5.3
作者：
Gersner, Roman;Kravetz, Elena;Zangen, Abraham
通讯作者：
Zangen, Abraham
伤害后塑性的分子神经影像学。
DOI：
10.1007/s12031-014-0347-y
发表时间：
2014-12
期刊：
Journal of molecular neuroscience : MN
影响因子：
--
作者：
Jouroukhin Y;Nonyane BA;Gilad AA;Pelled G
通讯作者：
Pelled G
影响因子：
5.3
作者：
Karl, A;Birbaumer, N;Flor, H
通讯作者：
Flor, H
DOI：
10.1038/nrn.2015.8
发表时间：
2016-01
期刊：
Nature reviews. Neuroscience
影响因子：
--
作者：
Kalueff AV;Stewart AM;Song C;Berridge KC;Graybiel AM;Fentress JC
通讯作者：
Fentress JC