Differences in airborne stability of SARS-CoV-2 variants of concern is impacted by alkalinity of surrogates of respiratory aerosol.
Differences in airborne stability of SARS-CoV-2 variants of concern is impacted by alkalinity of surrogates of respiratory aerosol.
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DOI：
10.1098/rsif.2023.0062
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发表时间：
2023-06
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
被假设为气溶胶阶段病毒感染性丧失的关键驱动因素的机械因素通常仍然是推测性的。使用下一代生物气溶胶技术，我们报告的空气动力学稳定性的几个SARS-CoV-2变异的关注气溶胶液滴的定义明确的大小和组成，在高（90%）和低（40%）相对湿度（RH）超过40分钟的测量。当与祖先的病毒相比，感染性的三角洲变体显示不同的衰减曲线。在低RH下，在最初的5 s内观察到两种变体的病毒感染性损失约55%。无论RH和变体如何，在雾化40分钟后，超过95%的病毒感染性丧失。这些变体的空气稳定性与其对碱性pH值的敏感性相关。去除所有酸性蒸气会显着增加感染性衰减的速率，2分钟后损失90%，而添加硝酸蒸气则提高了空气稳定性。在人工唾液和生长培养基的液滴中观察到类似的空气动力学稳定性。提出了一个预测病毒感染性丧失的模型：在高相对湿度下，呼出气溶胶的高pH值导致病毒感染性丧失;在低相对湿度下，高盐含量限制了病毒感染性的丧失。
The mechanistic factors hypothesized to be key drivers for the loss of infectivity of viruses in the aerosol phase often remain speculative. Using a next-generation bioaerosol technology, we report measurements of the aero-stability of several SARS-CoV-2 variants of concern in aerosol droplets of well-defined size and composition at high (90%) and low (40%) relative humidity (RH) upwards of 40 min. When compared with the ancestral virus, the infectivity of the Delta variant displayed different decay profiles. At low RH, a loss of viral infectivity of approximately 55% was observed over the initial 5 s for both variants. Regardless of RH and variant, greater than 95% of the viral infectivity was lost after 40 min of being aerosolized. Aero-stability of the variants correlate with their sensitivities to alkaline pH. Removal of all acidic vapours dramatically increased the rate of infectivity decay, with 90% loss after 2 min, while the addition of nitric acid vapour improved aero-stability. Similar aero-stability in droplets of artificial saliva and growth medium was observed. A model to predict loss of viral infectivity is proposed: at high RH, the high pH of exhaled aerosol drives viral infectivity loss; at low RH, high salt content limits the loss of viral infectivity.
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影响因子：
3.4
作者：
Courtney JM;Bax A
通讯作者：
Bax A
DOI：
10.3390/v14091856
发表时间：
2022-08-24
期刊：
Viruses
影响因子：
--
作者：
Alexander RW;Tian J;Haddrell AE;Oswin HP;Neal E;Hardy DA;Otero-Fernandez M;Mann JFS;Cogan TA;Finn A;Davidson AD;Hill DJ;Reid JP
通讯作者：
Reid JP
DOI：
10.1073/pnas.2109750119
发表时间：
2022-01-25
影响因子：
11.1
作者：
Huynh E;Olinger A;Woolley D;Kohli RK;Choczynski JM;Davies JF;Lin K;Marr LC;Davis RD
通讯作者：
Davis RD
影响因子：
3.8
作者：
BENBOUGH, JE
通讯作者：
BENBOUGH, JE
DOI：
10.1073/pnas.1902840116
发表时间：
2019-05-28
影响因子：
11.1
作者：
Kudo, Eriko;Song, Eric;Iwasaki, Akiko
通讯作者：
Iwasaki, Akiko