Identification of novel miRNAs and miRNA expression profiling in wheat hybrid necrosis.
Identification of novel miRNAs and miRNA expression profiling in wheat hybrid necrosis.
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小麦杂交坏死中新型 miRNA 的鉴定和 miRNA 表达谱分析
DOI：
10.1371/journal.pone.0117507
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Zhou J;Cheng Y;Yin M;Yang E;Gong W;Liu C;Zheng X;Deng K;Ren Z;Zhang Y
微小RNA（miRNA）在植物的生长和发育、防御病毒感染以及对环境变化的反应等众多生物过程中发挥着重要作用。小麦杂种坏死病是一种常见的遗传现象，它是致死或半致死的，导致杂种逐渐死亡或丧失生产力。然而，与小麦杂种坏死相关的分子基础和机制仍不清楚。在这里，我们报告的人口和表达谱的miRNAs在小麦杂交坏死。我们共鉴定了57个保守的miRNA家族以及182个推定的新miRNA。表达谱分析显示，49个已知的miRNAs和165个新的miRNAs的表达在杂交坏死中发生了变化。部分miRNAs及其预测靶点的表达水平已通过qRT-PCR得到证实。这些结果表明，这些miRNAs，特别是miR 159，miR 166，miR 167和miR 5072可能参与了广泛的基因表达调控，以响应杂交坏死。
MicroRNAs (miRNAs) play essential roles in a vast array of biological processes, including growth and development, defense against viral infection, and responses to environmental changes in plant. Wheat hybrid necrosis is an interesting genetic phenomenon observed frequency and it is lethal or semi lethal, resulting in gradual death or loss of productivity. However, the molecular basis and mechanisms associated with hybrid necrosis in wheat are still not well understood. Here, we report the population and expression profiles of miRNAs in wheat hybrid necrosis. We identified a total of 57 conserved miRNA families as well as 182 putative novel miRNAs. Expression profiling revealed that expression of 49 known miRNAs and 165 novel miRNAs was changed in hybrid necrosis. And the expression levels of some miRNAs and their predicated targets have been confirmed by qRT-PCR. These results indicate that these miRNAs, especially miR159, miR166, miR167 and miR5072 could be involved in the extensive regulation of gene expression in response to hybrid necrosis.
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影响因子：
14.9
作者：
Kozomara A;Griffiths-Jones S
通讯作者：
Griffiths-Jones S
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
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