A novel plasma circular RNA circFARSA is a potential biomarker for non-small cell lung cancer.
A novel plasma circular RNA circFARSA is a potential biomarker for non-small cell lung cancer.
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DOI：
10.1002/cam4.1514
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发表时间：
2018-06
期刊：
影响因子：
4
通讯作者：
Shen H
中科院分区：
文献类型：
--
作者：
Hang D;Zhou J;Qin N;Zhou W;Ma H;Jin G;Hu Z;Dai J;Shen H
新出现的证据表明环状rna （circRNAs）与癌症的发展有关。本研究旨在评估循环环状rna是否可以作为非小细胞肺癌（NSCLC）的新型生物标志物。我们使用RNA测序（RNA - seq）和定量实时PCR来探索与癌症相关的环状RNA。通过生物信息学和功能分析揭示环状rna对肺癌细胞的生物学作用。通过总RNA - seq共鉴定出5471个不同的环状RNA，其中185个在癌组织和邻近正常组织之间存在差异表达。来源于FARSA基因外显子5-7的circRNA被称为circFARSA，在癌组织中被观察到增加（P = 0.016），并且在患者血浆中比对照组更丰富（P < 0.001）。在A549细胞系中过表达circFARSA可显著促进细胞迁移和侵袭。硅分析表明circFARSA可能会吸收miR‐330‐5p和miR‐326，从而减轻它们对致癌基因脂肪酸合成酶的抑制作用。总之，本研究首次揭示了非小细胞肺癌的circRNA谱，并提供了血浆cirfarsa作为这种恶性肿瘤潜在的无创生物标志物的证据。
Emerging evidence indicates that circular RNAs (circRNAs) are implicated in cancer development. This study aimed to evaluate whether circulating circRNAs may serve as novel biomarkers for non‐small cell lung cancer (NSCLC). We used RNA sequencing (RNA‐seq) and quantitative real‐time PCR to explore cancer‐related circRNAs. Bioinformatics and functional analyses were performed to reveal biological effects of circRNAs on lung cancer cells. A total of 5471 distinct circRNAs were identified by total RNA‐seq, in which 185 were differentially expressed between cancerous and adjacent normal tissues. A circRNA derived from exon 5–7 of the FARSA gene, termed circFARSA, was observed to increase in cancerous tissues (P = 0.016), and was more abundant in patients’ plasma than controls (P < 0.001). Overexpression of circFARSA in A549 cell line significantly promoted cell migration and invasion. In silico analysis suggested that circFARSA might sponge miR‐330‐5p and miR‐326, thereby relieving their inhibitory effects on oncogene fatty acid synthase. Summarily, this study revealed circRNA profile of NSCLC for the first time and provided the evidence of plasma circFARSA as a potential noninvasive biomarker for this malignancy.
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影响因子：
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作者：
Li F;Zhang L;Li W;Deng J;Zheng J;An M;Lu J;Zhou Y
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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作者：
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通讯作者：
BAILLEUL, B
影响因子：
254.7
作者：
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通讯作者：
Jemal, Ahmedin