Fluorescent carbon dots as an efficient siRNA nanocarrier for its interference therapy in gastric cancer cells.
Fluorescent carbon dots as an efficient siRNA nanocarrier for its interference therapy in gastric cancer cells.
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荧光碳点作为有效的 siRNA 纳米载体，用于胃癌细胞的干扰治疗
DOI：
10.1186/s12951-014-0058-0
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发表时间：
2014-12-30
影响因子：
10.2
通讯作者：
Cui D
中科院分区：
文献类型：
--
作者：
Wang Q;Zhang C;Shen G;Liu H;Fu H;Cui D
背景荧光碳点（Cdot）由于其在传感、催化和生物医学等方面的潜在应用而受到越来越多的关注。目前，集中的研究已经集中在这些良性光致发光材料的合成和成像引导治疗。同时，Cdots已被探索作为非病毒载体的核酸或药物输送通过化学修饰上purpose.ResultsWe已经开发了微波辅助一步合成Cdots与柠檬酸为碳源和色氨酸（Trp）作为氮源和钝化剂。具有均匀尺寸的Cdot显示出上级水溶性、优异的生物相容性和高量子产率。然后将吸附有聚乙烯亚胺（PEI）的Cdots纳米颗粒（Cdots@PEI）用于将Survivin siRNA导入人胃癌细胞系MGC-803。结果证实，纳米载体表现出优异的生物相容性和显著增加siRNA的细胞递送，诱导Survivin蛋白的有效敲低至6.1%。此外，PEI@Cdots复合物可介导Survivin基因沉默，使细胞周期阻滞在G1期，并诱导细胞凋亡。结论Cdots复合物和PEI吸附复合物可作为显像剂和siRNA纳米载体用于Survivin siRNA的载体。结果表明，基于Cdots的纳米载体可以用于广泛的siRNA递送系统用于癌症治疗。
BackgroundFluorescent carbon dots (Cdots) have attracted increasing attention due to their potential applications in sensing, catalysis, and biomedicine. Currently, intensive research has been concentrated on the synthesis and imaging-guided therapy of these benign photoluminescent materials. Meanwhile, Cdots have been explored as nonviral vector for nucleic acid or drug delivery by chemical modification on purpose.ResultsWe have developed a microwave assisted one-step synthesis of Cdots with citric acid as carbon source and tryptophan (Trp) as both nitrogen source and passivation agent. The Cdots with uniform size show superior water solubility, excellent biocompatibility, and high quantum yield. Afterwards, the PEI (polyethylenimine)-adsorbed Cdots nanoparticles (Cdots@PEI) were applied to deliver Survivin siRNA into human gastric cancer cell line MGC-803. The results have confirmed the nanocarrier exhibited excellent biocompatibility and a significant increase in cellular delivery of siRNA, inducing efficient knockdown for Survivin protein to 6.1%. In addition, PEI@Cdots complexes mediated Survivin silencing, the arrested cell cycle progression in G1phase as well as cell apoptosis was observed.ConclusionThe Cdots-based and PEI-adsorbed complexes both as imaging agents and siRNA nanocarriers have been developed for Survivin siRNA delivery. And the results indicate that Cdots-based nanocarriers could be utilized in a broad range of siRNA delivery systems for cancer therapy.
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影响因子：
21.3
作者：
通讯作者：
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影响因子：
3.4
作者：
Parhamifar, Ladan;Larsen, Anna K.;Moghimi, S. Moein
通讯作者：
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作者：
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作者：
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通讯作者：
Uludag, Hasan
影响因子：
38.3
作者：
Lu, Jiong;Yeo, Pei Shan Emmeline;Loh, Kian Ping
通讯作者：
Loh, Kian Ping