Differentiation of Gastric Helicobacter Species Using MALDI-TOF Mass Spectrometry.
Differentiation of Gastric Helicobacter Species Using MALDI-TOF Mass Spectrometry.
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DOI：
10.3390/pathogens10030366
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发表时间：
2021-03-18
期刊：
影响因子：
--
通讯作者：
Haesebrouck F
中科院分区：
文献类型：
--
作者：
Berlamont H;De Witte C;De Bruyckere S;Fox JG;Backert S;Smet A;Boyen F;Haesebrouck F
胃幽门螺杆菌（Helicobacter pylori）和非幽门螺杆菌(non-H。幽门螺杆菌物种（NHPHs）在人类和/或动物的胃中定植。幽门螺杆菌的种类鉴定是必不可少的，因为其中许多被认为是人类和/或动物病原体。目前，幽门螺杆菌的种类只能通过分子方法进行区分。使用MALDI-TOF质谱区分nhph之前没有描述过，可能是因为这些物种在当前的MALDI-TOF质谱数据库中代表性不足。因此，我们利用MALDI-TOF质谱技术鉴定了10种不同幽门螺杆菌的93株胃幽门螺杆菌，以建立更完善的幽门螺杆菌参考数据库。虽然MALDI Biotyper数据库不能正确识别任何分离物，但内部数据库正确识别了所有单个质谱，并基于两个最高对数分数匹配（对数分数≥2）得出82%的正确物种鉴定。此外，利用所有新创建的主谱图构建了树状图。形成了9个主要的幽门螺杆菌群，一些在系统发育上密切相关的幽门螺杆菌种紧密聚集在一起，在特定种中观察到明确的亚群。目前的结果表明，只要有一个广泛的数据库，并且考虑到生长条件和琼脂培养基相关峰的变化，MALDI-TOF MS可以快速区分胃幽门螺杆菌种类。
Gastric helicobacters (Helicobacter (H.) pylori and non-H. pylori Helicobacter species (NHPHs)) colonize the stomach of humans and/or animals. Helicobacter species identification is essential since many of them are recognized as human and/or animal pathogens. Currently, Helicobacter species can only be differentiated using molecular methods. Differentiation between NHPHs using MALDI-TOF MS has not been described before, probably because these species are poorly represented in current MALDI-TOF MS databases. Therefore, we identified 93 gastric Helicobacter isolates of 10 different Helicobacter species using MALDI-TOF MS in order to establish a more elaborate Helicobacter reference database. While the MALDI Biotyper database was not able to correctly identify any of the isolates, the in-house database correctly identified all individual mass spectra and resulted in 82% correct species identification based on the two highest log score matches (with log scores ≥2). In addition, a dendrogram was constructed using all newly created main spectrum profiles. Nine main clusters were formed, with some phylogenetically closely related Helicobacter species clustering closely together and well-defined subclusters being observed in specific species. Current results suggest that MALDI-TOF MS allows rapid differentiation between gastric Helicobacter species, provided that an extensive database is at hand and variation due to growth conditions and agar-medium-related peaks are taken into account.
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DOI：
10.1099/00207713-49-4-1707
发表时间：
1999-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
De Groote, D;van Doorn, LJ;Vandamme, P
通讯作者：
Vandamme, P
影响因子：
9.4
作者：
Chen, Jonathan H. K.;Ho, Pak-Leung;Yam, Wing-Cheong
通讯作者：
Yam, Wing-Cheong
影响因子：
9.4
作者：
Harper, CG;Whary, MT;Fox, JG
通讯作者：
Fox, JG
影响因子：
2.4
作者：
Bokma, Jade;Pardon, Bart;Boyen, Filip
通讯作者：
Boyen, Filip
影响因子：
3.3
作者：
Berlamont, H.;Smet, A.;De Witte, C.
通讯作者：
De Witte, C.