Three-dimensional diffusion tensor microimaging for anatomical characterization of the mouse brain.
Three-dimensional diffusion tensor microimaging for anatomical characterization of the mouse brain.
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DOI：
10.1002/mrm.22426
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发表时间：
2010-07
影响因子：
3.3
通讯作者：
Zhang, Jiangyang
中科院分区：
文献类型：
--
作者：
Aggarwal, Manisha;Mori, Susumu;Shimogori, Tomomi;Blackshaw, Seth;Zhang, Jiangyang
扩散张量成像（DTI）对于发育中小鼠大脑的解剖成像越来越重要。然而，DTI在微观水平上对小鼠脑成像的应用受到可实现的空间分辨率的限制的阻碍。在这项研究中，快速扩散张量显微成像（DTMI）的基础上扩散加权梯度和自旋回波（DW-GRASE）技术与双导航回波相位校正的小鼠大脑。与回波平面和自旋回波采集相比，DW-GRASE采集显著缩短了扫描时间，并具有最小的图像失真，从而允许以更高的空间分辨率进行采集。在这项研究中，空间分辨率为50 - 60 µm的小鼠大脑三维DTMI揭示了前所未有的解剖细节。首次观察到成年小鼠纹状体中的细纤维束和胚胎12天小鼠脑中的白色物质束。该研究表明，使用DTMI技术获得的数据允许基因表达数据的三维映射，并且可以作为在发育中的小鼠大脑的神经解剖学背景下研究基因表达模式的平台。
Diffusion tensor imaging (DTI) is gaining increasing importance for anatomical imaging of the developing mouse brain. However, the application of DTI to mouse brain imaging at microscopic levels is hindered by the limitation on achievable spatial resolution. In this study, fast diffusion tensor microimaging (DTMI) of the mouse brain based on a diffusion-weighted gradient and spin echo (DW-GRASE) technique with twin-navigator echo phase correction is presented. Compared to echo planar and spin echo acquisition, the DW-GRASE acquisition resulted in significant reduction in scan time and had minimal image distortion, thereby allowing acquisition at higher spatial resolution. In this study, three dimensional DTMI of the mouse brains at spatial resolutions of 50 – 60 µm revealed unprecedented anatomical details. Thin fiber bundles in the adult striatum and white matter tracts in the embryonic day 12 mouse brains were visualized for the first time. The study demonstrated that data acquired using the DTMI technique allow three dimensional mapping of gene expression data and can serve as a platform to study gene expression patterns in the context of neuroanatomy in the developing mouse brain.
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影响因子：
5.7
作者：
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通讯作者：
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3
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作者：
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影响因子：
2.4
作者：
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