Role of diabetes in lung injury from acute exposure to electronic cigarette, heated tobacco product, and combustible cigarette aerosols in an animal model.
Role of diabetes in lung injury from acute exposure to electronic cigarette, heated tobacco product, and combustible cigarette aerosols in an animal model.
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DOI：
10.1371/journal.pone.0255876
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Husari A
中科院分区：
文献类型：
--
作者：
Daou MAZ;Shihadeh A;Hashem Y;Bitar H;Kassir A;El-Harakeh M;Karaoghlanian N;Eid AA;El-Sabban M;Zaatari G;Husari A
与普通人群相比，糖尿病患者更容易受到可燃香烟烟雾（CS）的有害呼吸影响。电子烟（ECIG）和加热烟草产品（HTP）作为CS的危害较小的替代品销售。在这项研究中，我们在动物模型中比较了急性ECIG，HTP和CS暴露对II型糖尿病小鼠和非糖尿病小鼠肺部的影响。将II型糖尿病（Diab）和非糖尿病（Non-Diab）小鼠分为对照组、ECIG组、HTP组和CS组。动物暴露6小时。每天进行空中、ECIG、HTP或CS，持续七天。通过a）组织病理学，B）湿干比，c）支气管肺泡灌洗液中的白蛋白浓度，d）TNF-α、IL-6和IL-1 β的表达，e）活性氧产生（ROS）和f）细胞凋亡评估来确定肺损伤。肺组织学显示暴露于ECIG、HTP和CS的糖尿病小鼠的水肿和炎性细胞增加。炎症介质的表达在糖尿病组中也更显著。例如，TNF-α表达在Diab + ECIG中上调，但在非Diab + ECIG中未上调。Diab + CS组ROS显著增加，Non-Diab + CS组ROS较少，ECIG + Diab组ROS较弱。在Diab和非Diab HTP暴露动物中观察到显著白蛋白渗漏。与非Diab小鼠相比，CS暴露加重了Diab小鼠的肺损伤。糖尿病等合并症可能会放大CS、ECIG或HTP暴露的不良影响。
Patients with diabetes are more vulnerable to the detrimental respiratory effects of combustible cigarette smoke (CS) when compared to the general population. Electronic cigarettes (ECIG) and heated tobacco products (HTP) are marketed as less harmful alternatives to CS. In this study, we compared the effects of acute ECIG, HTP and CS exposure on the lungs of type II diabetes versus non-diabetic mice in an animal model. Type II Diabetic (Diab) and Non-Diabetic (Non-Diab) mice were divided into Control, ECIG, HTP and CS groups. Animals were exposed for 6 hrs./day to either air, ECIG, HTP or CS for seven days. Lung injury was determined by a) histopathology, b) wet to dry ratio, c) albumin concentration in bronchoalveolar lavage fluid, d) expression of TNF-α, IL-6, and IL-1 β, e) reactive oxygen species production (ROS), and f) assessment of cellular apoptosis. Lung histology revealed increased edema and inflammatory cells in diabetic mice exposed to ECIG, HTP and CS. The expression of Inflammatory mediators was, in general, more significant in the Diabetic groups as well. TNF-α expression, for example, was upregulated in Diab + ECIG but not in Non-Diab + ECIG. ROS was significantly increased in Diab + CS, less in Non-Diab + CS and weakly noted in ECIG + Diab. Significant albumin leak was observed in Diab and Non-Diab HTP-exposed animals. CS exposure worsened lung injury in Diab when compared to Non-Diab mice. Comorbid medical conditions like diabetes may amplify ill effects of CS, ECIG or HTP exposure.
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影响因子：
5.2
作者：
Glantz SA
通讯作者：
Glantz SA
影响因子：
3.2
作者：
Putcha N;Drummond MB;Wise RA;Hansel NN
通讯作者：
Hansel NN
影响因子：
24.3
作者：
Mannino, D. M.;Thorn, D.;Holguin, F.
通讯作者：
Holguin, F.
DOI：
10.4331/wjbc.v8.i2.120
发表时间：
2017-05-26
期刊：
World journal of biological chemistry
影响因子：
--
作者：
Pirola L;Ferraz JC
通讯作者：
Ferraz JC
影响因子：
4.7
作者：
Talih, Soha;Balhas, Zainab;Shihadeh, Alan
通讯作者：
Shihadeh, Alan