Platelets convert peripheral blood circulating monocytes to regulatory cells via immunoglobulin G and activating-type Fcγ receptors.
Platelets convert peripheral blood circulating monocytes to regulatory cells via immunoglobulin G and activating-type Fcγ receptors.
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DOI：
10.1186/s12865-015-0086-z
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发表时间：
2015-04-21
期刊：
影响因子：
3
通讯作者：
Takai T
中科院分区：
文献类型：
--
作者：
Inui M;Tazawa K;Kishi Y;Takai T
单核细胞和巨噬细胞产生白细胞介素（IL）-10，一种免疫调节细胞因子和免疫疾病的有效治疗工具。通过用toll样受体配体和免疫球蛋白（IG）G免疫复合物双重刺激细胞，获得体外巨噬细胞中IL-10产生的增加和促炎细胞因子的伴随减少，该反应被称为调节性（或可替代地活化的/M2）巨噬细胞的反应。然而，尚未充分探索如何利用这种调节反应用于抗炎。我们的目的是寻找一种潜在的方式或条件，通过单核/巨噬细胞在体内和体外增加IL-10。我们表明，当血小板被IgG调理时，可以在体外和小鼠体内模型中将人外周血循环单核细胞转化为产生IL-10的调节性单核细胞。在抗整合素IgG和细菌脂多糖的存在下，血小板和单核细胞的共培养通过血小板和单核细胞之间的直接相互作用增强IL-10的产生。这种增强IL-10的新方式是由IgG的激活型Fc受体介导的。这些发现表明IgG结合的血小板诱导的单核细胞向调节细胞的转化可能提供控制炎症的新策略。本文的在线版本（doi：10.1186/s12865-015-0086-z）包含补充材料，可供授权用户使用。
Monocytes and macrophages produce interleukin (IL)-10, an immunoregulatory cytokine and a potent therapeutic tool for immune disorders. Augmentation of IL-10 production with a concomitant reduction of proinflammatory cytokines in macrophages in vitro is attained by doubly stimulating the cells with a toll-like receptor ligand and immunoglobulin (Ig)G immune complexes, a response known as that of regulatory (or alternatively activated/M2) macrophages. However, it has not been explored sufficiently how such a regulatory response could be exploited for anti-inflammation. Our objective is to find a potential way or condition for augmenting IL-10 by monocytes/macrophages in vivo and in vitro. We show that platelets, when they are opsonized with IgG, can convert human peripheral blood circulating monocytes to IL-10-producing regulatory monocytes in vitro and also in a murine in vivo model. Co-culturing of platelets and monocytes in the presence of anti-integrin IgG and a bacterial lipopolysaccharide augmented IL-10 production via a direct interaction between platelets and monocytes. This novel way of enhancing IL-10 was mediated by activating-type Fc receptors for IgG. These findings indicate that the IgG-bound platelet-induced conversion of monocytes to regulatory cells might provide a novel strategy for controlling inflammation. The online version of this article (doi:10.1186/s12865-015-0086-z) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
Gerber, JS;Mosser, DM
通讯作者：
Mosser, DM
DOI：
10.4049/jimmunol.1300649
发表时间：
2013-09-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Maseda D;Candando KM;Smith SH;Kalampokis I;Weaver CT;Plevy SE;Poe JC;Tedder TF
通讯作者：
Tedder TF
影响因子：
64.5
作者：
TAKAI, T;LI, M;RAVETCH, JV
通讯作者：
RAVETCH, JV
IgG免疫复合物在感染Leishmania的感染过程中的作用。
影响因子：
15.3
作者：
Miles, SA;Conrad, SM;Aves, RG;Jeronimo, SMB;Mosser, DM
通讯作者：
Mosser, DM
DOI：
10.1084/jem.170.6.2081
发表时间：
1989-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Fiorentino DF;Bond MW;Mosmann TR
通讯作者：
Mosmann TR