Low temperature culture enhances ameloblastic differentiation of human keratinocyte stem cells
Low temperature culture enhances ameloblastic differentiation of human keratinocyte stem cells
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低温培养增强人角质形成细胞干细胞的成釉细胞分化
DOI：
10.1007/s10735-019-09837-9
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发表时间：
2019-07
影响因子：
3.2
通讯作者：
ing
中科院分区：
文献类型：
--
作者：
Song Yingnan;Wang Bingmei;Li Hua;Hu Xiaoxiao;Lin Xin;Hu Xuefeng;Zhang Y;ing
先前的研究表明，几种类型的非牙源性人类干细胞在与小鼠胚胎牙间充质重组后，可以被诱导分化为分泌釉质的成釉细胞。然而，成釉细胞分化的成功率较低，这是未来以干细胞为基础的全牙生物工程的主要障碍。此前的研究表明，在组织工程中，低温培养可以提高干细胞的分化能力。在本研究中，我们系统地研究了低温对体外培养的人角质形成细胞干细胞(HKSCs)的活性、增殖和干性的影响，并进一步研究了hKSCs在人-鼠重组嵌合牙胚中的成釉分化。我们的结果表明，低温确实降低了hKSCs的生长速度，并保持了健康的未分化形态，而对细胞活力没有任何影响。此外，与37℃培养的hKSCs相比，低温培养的hKSCs的干性得到了改善，干性标记K15、CD29和P63的表达增加，而表达分化标记K10的表达减少。这些低温处理的hKSCs与小鼠胚胎牙间充质细胞重组后，成釉细胞分化率(40%)显著高于标准温度处理的hKSCs组织重组的成釉细胞分化率(17%)。我们的研究表明，低温细胞培养提高了hKSCs的干性和可塑性，进而增强了生物工程牙齿干细胞的成釉分化能力。
Previous studies have demonstrated that several types of human stem cells of non-dental origin can be induced to differentiate into enamel-secreting ameloblasts after recombined with mouse embryonic dental mesenchyme. However, the successful rate of ameloblastic differentiation is about rather low, which presents a major obstacle for future stem cell-based whole tooth bioengineering. Previous studies have shown that cultures at reduced temperature could improve the differentiation capability of stem cells in tissue engineering. In this study, we systematically investigated the effects of low temperature on the viability, proliferation and stemness of human keratinocytes stem cells (hKSCs) in cell culture and further examined ameloblastic differentiation of the hKSCs in human–mouse recombinant chimeric tooth germs. Our results demonstrated that low temperature indeed reduces growth rate and maintains healthy undifferentiated morphology of hKSCs without any effects on cell viability. Moreover, examination of stemness makers revealed improved stemness of hKSCs cultured at low temperature with increased expression of stemness markers K15, CD29 and p63 and decreased expression differentiation marker K10, as compared to those cultured at 37 °C. These low temperature treated hKSCs, when recombined with mouse embryonic dental mesenchyme, exhibited significantly increased rate (40%) of ameloblastic differentiation, as compared to that (17%) in tissue recombinants with those hKSCs treated at standard temperature. Our studies demonstrate that low temperature cell culture improves the stemness and plasticity of hKSCs, which in turn enhances ameloblastic differentiation capability of the stem cells in bioengineered teeth.
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影响因子：
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作者：
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影响因子：
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DOI：
10.1186/2045-9769-2-6
发表时间：
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期刊：
Cell regeneration (London, England)
影响因子：
--
作者：
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通讯作者：
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影响因子：
4.3
作者：
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通讯作者：
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