Cell Structure Controls Endothelial Cell Migration under Fluid Shear Stress.
Cell Structure Controls Endothelial Cell Migration under Fluid Shear Stress.
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DOI：
10.1007/s12195-009-0060-z
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发表时间：
2009-06-01
影响因子：
2.8
通讯作者：
Helmke, Brian P.
中科院分区：
文献类型：
--
作者：
Lin, Xiefan;Helmke, Brian P.
融合单层中的鹅卵石状内皮细胞在稳定的单向剪应力作用下经历三相机械趋向性，但在微图案基质上拉长并排列的细胞不会改变其迁移行为，以响应垂直或平行流动。微图案化内皮细胞层的机械趋向性是否被细长的细胞骨架结构或有限的粘附区所抑制尚不清楚。在这项研究中，细胞在剪切应力开始后在宽(100-200μm)微图案线上进行检查。线条中心区域的细胞呈现鹅卵石形态和类似于未图案化单层的三相机械趋向性行为，而沿边缘的细胞平行于线轴迁移，而与流动方向无关。当垂直于微纹纹线产生划痕伤口时，细胞在迁移到裸露区域之前变得不那么细长。在垂直于流动方向的稀疏线中，细长细胞沿上游边缘平行迁移7h，然后平行于剪应力方向迁移，尽管下游方向存在粘附区。因此，细胞骨架结构和不可用的粘附区是决定内皮细胞机械性趋化是否发生对剪应力的响应的主要因素。
Cobblestone-shaped endothelial cells in confluent monolayers undergo triphasic mechanotaxis in response to steady unidirectional shear stress, but cells that are elongated and aligned on micropatterned substrates do not change their migration behavior in response to either perpendicular or parallel flow. Whether mechanotaxis of micropatterned endothelial cell layers is suppressed by elongated cytoskeletal structure or limited availability of adhesion area remains unknown. In this study, cells were examined on wide (100–200 μm) micropatterned lines after onset of shear stress. Cells in center regions of the lines exhibited cobblestone morphology and triphasic mechanotaxis behavior similar to that in unpatterned monolayers, whereas cells along the edges migrated parallel to the line axis regardless of the flow direction. When scratch wounds were created perpendicular to the micropatterned lines, the cells became less elongated before migrating into the denuded area. In sparsely populated lines oriented perpendicular to the flow direction, elongated cells along the upstream edge migrated parallel to the edge for 7 h before migrating parallel to the shear stress direction, even though adhesion area existed in the downstream direction. Thus, cytoskeletal structure and not available adhesion area serves as the dominant factor in determining whether endothelial mechanotaxis occurs in response to shear stress.
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DOI：
10.1115/1.3138276
发表时间：
1981-01-01
影响因子：
1.7
作者：
DEWEY, CF;BUSSOLARI, SR;DAVIES, PF
通讯作者：
DAVIES, PF
影响因子：
5.5
作者：
Mott, Rosalind E.;Helmke, Brian P.
通讯作者：
Helmke, Brian P.
DOI：
10.1161/01.atv.10.2.215
发表时间：
1990-03-01
期刊：
ARTERIOSCLEROSIS
影响因子：
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作者：
COOMBER, BL;GOTLIEB, AI
通讯作者：
GOTLIEB, AI
DOI：
10.1016/j.bbrc.2005.08.272
发表时间：
2005-11-11
影响因子：
3.1
作者：
Hsu, S;Thakar, R;Li, S
通讯作者：
Li, S
DOI：
10.1115/1.1486468
发表时间：
2002-08-01
影响因子：
1.7
作者：
Blackman, BR;García-Cardeña, G;Gimbrone, MA
通讯作者：
Gimbrone, MA