Type IV pilus retraction is required for Neisseria musculi colonization and persistence in a natural mouse model of infection.
Type IV pilus retraction is required for Neisseria musculi colonization and persistence in a natural mouse model of infection.
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致病性奈瑟氏菌IV型菌毛（Tfp）的缩回需要主要缩回马达PilT及其旁系同源物皮鲁和PilT 2。Tfp回缩对自然感染的重要性尚不清楚。使用我们的天然动物模型的奈瑟氏球菌宿主相互作用，我们已经研究了这些蛋白质的作用，在肠道的肌肉奈瑟氏球菌（Nmus）的能力，殖民和坚持在其原生宿主，小鼠。我们报道了Nmus ΔpilT不能定殖于小鼠; Δ皮鲁U和Δ pilT 2可以定殖并持续存在于小鼠中，但数量较少。由ΔpilT、ΔpilTU和pilTL 201 C形成的小菌落，表达在其ATP水解结构域中具有点突变的PilT，对流体剪切力的去除敏感。因此，PilT促进Nmus定殖，而皮鲁和PilT 2影响持久性。我们提出了一个非排他性的模型，这些TFP收缩马达蛋白如何有助于Nmus的持续定植。我们的研究结果有意义的作用，这些马达蛋白介导的相互作用，人类适应的致病性和肠道奈瑟菌与人类宿主。我们描述了IV型菌毛回缩对小鼠肠道奈瑟氏球菌（Neisseria，N。musculi，在其原生宿主中。我们的研究结果有影响的作用TFP收缩介导的相互作用的人类适应的致病性和肠道奈瑟氏菌与人类宿主由于这些物种的相关性。
Retraction of the pathogenic Neisseria Type IV pilus (Tfp) requires PilT, the primary retraction motor, and its paralogs PilU and PilT2. The importance of Tfp retraction for natural infection is unknown. Using our natural animal model of Neisseria-host interaction, we have examined the role of these proteins in the ability of commensal Neisseria musculi (Nmus) to colonize and persist in its native host, the mouse. We report that Nmus ΔpilT cannot colonize mice; ΔpilU and ΔpilT2 can colonize and persist in mice, but in lower numbers. Microcolonies formed by ΔpilT, ΔpilTU, and pilTL201C, expressing PilT with a point mutation in its ATP hydrolysis domain, are sensitive to removal by fluid shear forces. Thus, PilT promotes Nmus colonization while PilU and PilT2 influence persistence. We present a non-exclusive model for how these Tfp retraction motor proteins contribute to Nmus persistent colonization. Our findings have implications for the roles of these motor proteins in mediating interactions of human-adapted pathogenic and commensal Neisseria with their human host. We describe the importance of Type IV pilus retraction to colonization and persistence by a mouse commensal Neisseria, N. musculi, in its native host. Our findings have implications for the role of Tfp retraction in mediating interactions of human-adapted pathogenic and commensal Neisseria with their human host due to the relatedness of these species.
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影响因子：
--
作者：
Dillard JP
通讯作者：
Dillard JP
DOI：
10.1126/science.aad2001
发表时间：
2016-03-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chang YW;Rettberg LA;Treuner-Lange A;Iwasa J;Søgaard-Andersen L;Jensen GJ
通讯作者：
Jensen GJ
影响因子：
3.5
作者：
Abraham A;Pedregosa F;Eickenberg M;Gervais P;Mueller A;Kossaifi J;Gramfort A;Thirion B;Varoquaux G
通讯作者：
Varoquaux G
影响因子：
56.9
作者：
Chamot-Rooke, Julia;Mikaty, Guillain;Dumenil, Guillaume
通讯作者：
Dumenil, Guillaume
影响因子：
3.1
作者：
Eriksson, Jens;Eriksson, Olaspers Sara;Jonsson, Ann-Beth
通讯作者：
Jonsson, Ann-Beth