Development of a sensitive method to extract and detect low numbers of Cryptosporidium oocysts from adult cattle faecal samples.
Development of a sensitive method to extract and detect low numbers of Cryptosporidium oocysts from adult cattle faecal samples.
复制标题
开发一种从成年牛粪便样本中提取和检测少量隐孢子虫卵囊的灵敏方法。
DOI：
10.1016/j.vetpar.2016.07.018
复制
发表时间：
2016
影响因子：
2.6
通讯作者：
Wells B
中科院分区：
文献类型：
--
作者：
Wells B
迄今为止的隐孢子虫传播研究得出的结论是，成年牛不是导致农场犊牛临床隐孢子虫病的卵囊的重要来源。然而，目前的样品处理方法已针对小牛粪便样品进行了优化，并且由于卵囊数量较少和样品尺寸较大，因此在用于成人样品时可能不太敏感。一种改进的和新的卵囊提取和浓缩的方法被开发和应用于一项实验，涉及与已知浓度的隐孢子虫的成年牛粪便样品加标。结果表明，增加了检测的灵敏度从100卵囊/克粪便样品使用传统的协议，5卵囊/克使用新开发的方法。由于能够准确评估成年反刍动物对农场和环境中隐孢子虫传播的贡献非常重要，因此本文所述技术的发展可能会对未来隐孢子虫传播研究以及随后旨在减少农场小牛隐孢子虫感染的控制策略做出重要贡献。
Cryptosporidiumtransmission studies to date have concluded that adult cattle are not a significant source of oocysts contributing to clinical cryptosporidiosis in calves on farm. However current methods of sample processing have been optimised for calf faecal samples and may be less sensitive when used on adult samples due to lower numbers of oocysts and larger size of samples. A modified and novel method of oocyst extraction and concentration was developed and applied in an experiment involving spiking adult cattle faecal samples with known concentrations ofCryptosporidiumoocysts. The results showed an increased sensitivity of detection from 100 oocysts/g of faecal sample using conventional protocols to 5 oocysts/g using the newly developed method. As it is important to be able to accurately assess the contribution of adult ruminants to the transmission ofCryptosporidium,both on farm and in the environment, the development of the techniques described here is likely to make an important contribution toCryptosporidiumtransmission studies in future and in subsequent control strategies aimed at the reduction ofCryptosporidiuminfection in calves on farm.
登录
查看更多内容
法国西部奶牛群高分泌乳牛犊中隐孢子虫分离物的分子特征
影响因子：
2
作者：
A. Rieux;C. Chartier;I. Pors;A. Delafosse;C. Paraud
通讯作者：
C. Paraud
影响因子：
1.3
作者：
Atwill, ER;Pereira, MDC
通讯作者：
Pereira, MDC
影响因子：
4.4
作者：
Elwin, K;Chalmers, RM;Casemore, DP
通讯作者：
Casemore, DP
影响因子：
2.6
作者：
Smith RP;Clifton-Hadley FA;Cheney T;Giles M
通讯作者：
Giles M
影响因子：
1.5
作者：
MacLeod, A;Turner, CMR;Tait, A
通讯作者：
Tait, A