A point-of-care diagnostics logic detector based on glucose oxidase immobilized lanthanide functionalized metal-organic frameworks.
A point-of-care diagnostics logic detector based on glucose oxidase immobilized lanthanide functionalized metal-organic frameworks.
复制标题
基于葡萄糖氧化酶固定化镧系元素功能化金属有机框架的护理点诊断逻辑检测器。
DOI：
10.1039/c9nr06475c
复制
发表时间：
2019-11
期刊：
影响因子：
6.7
通讯作者：
Yan Bing
中科院分区：
文献类型：
--
作者：
Zhang Yu;Yan Bing
在这项工作中，首次通过Eu 3 +@UMOF和葡萄糖氧化酶（GOx）之间的表面附着策略制备了一种新型镧系元素功能化金属有机框架酶（L-MOF-酶）复合物。在这里，Eu 3 +@UMOF可以用作GOx固定的支持物，也可以用作对葡萄糖（Glu）的响应性荧光中心。该材料不仅具有Eu ~（3+）的5D 0 → 7 F2跃迁和酶的催化性能，而且具有MOF-酶复合物的一些优点，包括更好的稳定性，对Glu的荧光选择性和灵敏度（检测限为0.2 μM）。此外，该复合物在室温和中性条件下对血清和尿液中的Glu具有良好的选择性和灵敏度，突破了酶催化条件的限制。利用L-MOF-酶复合物的所有优点，设计了可用于尿液中Glu的荧光检测的床旁（POC）诊断逻辑检测器。从逻辑检测器的三个输出（L、M和H）中，我们可以直观地实现对作为检测器输入的三个Glu浓度范围（0.1 μM-10 μM、10 μM-10 mM、>10 mM）的肉眼自诊断。逻辑检测器可以让我们，特别是糖尿病患者，立即检测尿液中的葡萄糖水平，而无需去医院进行复杂的检查。这是首次尝试使用L-MOFs结合GOx构建用于荧光检测Glu的POC诊断逻辑检测器。
In this work, a novel lanthanide functionalized metal organic framework enzyme (L-MOF-enzyme) composite has been first prepared via a surface attachment strategy between Eu3+@UMOF and glucose oxidase (GOx). Here, the Eu3+@UMOF can be used as a support for GOx immobilization and also a responsive fluorescent center towards glucose (Glu). The resulting material not only exhibits fascinating luminescence properties based on the 5D0 → 7F2 transition of Eu3+ and the catalytic performance of enzymes, but also some advantages of MOF-enzyme composites, including better stability, and great fluorescence selectivity and sensitivity towards Glu (detection limit = 0.2 μM). Besides, the composite exhibited an excellent selectivity and sensitivity towards Glu in serum and urine under room temperature and neutral conditions, which breaks the limitation of specific catalytic conditions of enzymes. Taking all the advantages of the L-MOF-enzyme composite, a point-of care (POC) diagnostics logic detector which can be used for the fluorescence detection of Glu in urine is designed. From the three outputs of the logic detector (L, M and H), we can intuitively realize the self-diagnosis of the three ranges of Glu concentrations that act as the inputs of the detector (0.1 μM-10 μM, 10 μM-10 mM, >10 mM) by the naked eye. The logic detector allows us, especially diabetics, to instantly detect glucose levels in the urine without going to the hospital for complicated inspections. This is the first attempt using L-MOFs combined with GOx to construct a POC diagnostics logic detector for fluorescence detection of Glu.
登录
查看更多内容
基于 2D-MOF 纳米片的新型灵敏化学发光传感器，用于一步检测人尿液中的葡萄糖
影响因子：
3.7
作者：
Zhu Nuanfei;Gu Lantian;Wang Jin;Li Xuesong;Liang Guoxi;Zhou Jinhui;Zhang Zhen
通讯作者：
Zhang Zhen
通过使用 Cu-MOFs 一锅封装可回收的 β-葡萄糖苷酶，以增强纤维素水解为葡萄糖
影响因子：
8.4
作者：
Wang Lei;Zhi Wenjing;Wan Jing;Han Juan;Li Chunmei;Wang Yun
通讯作者：
Wang Yun
一种基于发光金属有机框架的便携式甲醛梯度自校准逻辑检测器
影响因子：
6.4
作者：
Zhang Yu;Yan Bing
通讯作者：
Yan Bing
纳米级荧光金属有机框架复合材料作为哮喘潜在诊断的逻辑平台
影响因子：
12.6
作者：
Zhang Xin;Fang Liquan;Jiang Ke;He Huajun;Yang Yu;Cui Yuanjing;Li Bin;Qian Guodong
通讯作者：
Qian Guodong
影响因子：
6.4
作者：
M. A. Kaloo;Ruchika Mishra;J. Sankar
通讯作者：
M. A. Kaloo;Ruchika Mishra;J. Sankar