Generation of induced pluripotent stem cells from human nasal epithelial cells using a Sendai virus vector.
Generation of induced pluripotent stem cells from human nasal epithelial cells using a Sendai virus vector.
复制标题
DOI：
10.1371/journal.pone.0042855
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Noguchi E
中科院分区：
文献类型：
--
作者：
Ono M;Hamada Y;Horiuchi Y;Matsuo-Takasaki M;Imoto Y;Satomi K;Arinami T;Hasegawa M;Fujioka T;Nakamura Y;Noguchi E
通过将重编程因子导入体细胞产生诱导多能干细胞(IPSCs)是再生医学中干细胞治疗的一种很有前途的方法。因此，开发一种微创的简单方法来制造IPSCs是可取的。在这项研究中，我们通过仙台病毒(SeV)载体的基因转导获得了人鼻腔上皮细胞(HNECs)来源的IPSCs。HNEC可以从受试者身上以非侵入性的方式获得，而不需要麻醉或活检。此外，SeV没有改变宿主基因组的风险，这在人类IPSCs的产生过程中提供了额外的安全水平。SeV感染的多样性为3~4，细胞重编程效率为0.08~0.10%。来源于HNECs的IPSCs具有与人类胚胎干细胞一致的全球基因表达谱和表观遗传状态。HNECs易于获得，再加上其强大的重编程特性，将为利用具有特定基因类型的细胞在体外研究疾病发病机制和分子机制提供机会。
The generation of induced pluripotent stem cells (iPSCs) by introducing reprogramming factors into somatic cells is a promising method for stem cell therapy in regenerative medicine. Therefore, it is desirable to develop a minimally invasive simple method to create iPSCs. In this study, we generated human nasal epithelial cells (HNECs)-derived iPSCs by gene transduction with Sendai virus (SeV) vectors. HNECs can be obtained from subjects in a noninvasive manner, without anesthesia or biopsy. In addition, SeV carries no risk of altering the host genome, which provides an additional level of safety during generation of human iPSCs. The multiplicity of SeV infection ranged from 3 to 4, and the reprogramming efficiency of HNECs was 0.08–0.10%. iPSCs derived from HNECs had global gene expression profiles and epigenetic states consistent with those of human embryonic stem cells. The ease with which HNECs can be obtained, together with their robust reprogramming characteristics, will provide opportunities to investigate disease pathogenesis and molecular mechanisms in vitro, using cells with particular genotypes.
登录
查看更多内容
影响因子：
64.5
作者：
Park IH;Arora N;Huo H;Maherali N;Ahfeldt T;Shimamura A;Lensch MW;Cowan C;Hochedlinger K;Daley GQ
通讯作者：
Daley GQ
影响因子：
64.5
作者：
Hanna, Jacob;Markoulaki, Styliani;Jaenisch, Rudolf
通讯作者：
Jaenisch, Rudolf
影响因子：
23.9
作者：
Loh YH;Hartung O;Li H;Guo C;Sahalie JM;Manos PD;Urbach A;Heffner GC;Grskovic M;Vigneault F;Lensch MW;Park IH;Agarwal S;Church GM;Collins JJ;Irion S;Daley GQ
通讯作者：
Daley GQ
影响因子：
56.9
作者：
Yu, Junying;Vodyanik, Maxim A.;Thomson, James A.
通讯作者：
Thomson, James A.
影响因子：
23.9
作者：
Seki, Tomohisa;Yuasa, Shinsuke;Fukuda, Keiichi
通讯作者：
Fukuda, Keiichi