Limited effects of long-term daily cranberry consumption on the gut microbiome in a placebo-controlled study of women with recurrent urinary tract infections.
Limited effects of long-term daily cranberry consumption on the gut microbiome in a placebo-controlled study of women with recurrent urinary tract infections.
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DOI：
10.1186/s12866-021-02106-4
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发表时间：
2021-02-18
期刊：
影响因子：
4.2
通讯作者：
Earl AM
中科院分区：
文献类型：
--
作者：
Straub TJ;Chou WC;Manson AL;Schreiber HL 4th;Walker BJ;Desjardins CA;Chapman SB;Kaspar KL;Kahsai OJ;Traylor E;Dodson KW;Hullar MAJ;Hultgren SJ;Khoo C;Earl AM
在美国，尿路感染（UTI）每年影响1500万女性，其中> 20%经历频繁复发的UTI。最近的一项安慰剂对照临床试验发现，在24周内每天饮用蔓越莓饮料的复发性UTI患者中，UTI症状减少了39%。使用来自这些试验参与者子集的粪便宏基因组测序，我们评估了蔓越莓消费对肠道微生物群的影响，肠道微生物群是导致UTI的病原体（如大肠杆菌）的储存库，导致> 80%的UTI。本文对包括大肠杆菌在内的绝大多数细菌类群的总体分类组成、群落多样性、功能途径和基因家族的携带以及相对丰度进行了研究。大肠杆菌，并没有改变显着的蔓越莓消费。然而，一种未命名的Flavonifractor物种（OTU 41），占总宏基因组的≤1%，在试验完成时，与安慰剂相比，在蔓越莓消费者中的丰度明显较低。鉴于Flavonifractor与负面人类健康影响的关联，我们试图确定OTU 41特征基因，这些基因可以解释其差异丰度和/或与关键宿主功能的关系。使用比较基因组和宏基因组技术，我们确定了OTU 41中与各种化合物（包括色氨酸和钴胺素）的运输和代谢相关的基因，这些化合物已被证明在宿主-微生物相互作用中发挥作用。虽然我们的研究结果表明，蔓越莓汁消费对微生物组的全球测量影响不大，但我们发现一种未命名的Flavonifractor物种在研究组之间存在显著差异。这表明需要进一步的研究来评估蔓越莓消费和Flavonifractor在复发性UTI背景下对健康和福祉的作用。临床试验注册号：ClinicalTrials.govNCT01776021。在线版本包含补充材料，可通过10.1186/s12866-021-02106-4获得。
Urinary tract infections (UTIs) affect 15 million women each year in the United States, with > 20% experiencing frequent recurrent UTIs. A recent placebo-controlled clinical trial found a 39% reduction in UTI symptoms among recurrent UTI sufferers who consumed a daily cranberry beverage for 24 weeks. Using metagenomic sequencing of stool from a subset of these trial participants, we assessed the impact of cranberry consumption on the gut microbiota, a reservoir for UTI-causing pathogens such as Escherichia coli, which causes > 80% of UTIs. The overall taxonomic composition, community diversity, carriage of functional pathways and gene families, and relative abundances of the vast majority of observed bacterial taxa, including E. coli, were not changed significantly by cranberry consumption. However, one unnamed Flavonifractor species (OTU41), which represented ≤1% of the overall metagenome, was significantly less abundant in cranberry consumers compared to placebo at trial completion. Given Flavonifractor’s association with negative human health effects, we sought to determine OTU41 characteristic genes that may explain its differential abundance and/or relationship to key host functions. Using comparative genomic and metagenomic techniques, we identified genes in OTU41 related to transport and metabolism of various compounds, including tryptophan and cobalamin, which have been shown to play roles in host-microbe interactions. While our results indicated that cranberry juice consumption had little impact on global measures of the microbiome, we found one unnamed Flavonifractor species differed significantly between study arms. This suggests further studies are needed to assess the role of cranberry consumption and Flavonifractor in health and wellbeing in the context of recurrent UTI. Clinical trial registration number: ClinicalTrials.govNCT01776021. The online version contains supplementary material available at 10.1186/s12866-021-02106-4.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
14.9
作者：
The Gene Ontology Consortium
通讯作者：
The Gene Ontology Consortium
影响因子：
15.1
作者：
Coello, Klara;Hansen, Tue Haldor;Vinberg, Maj
通讯作者：
Vinberg, Maj
影响因子：
3.7
作者：
Siddharth J;Holway N;Parkinson SJ
通讯作者：
Parkinson SJ
影响因子：
6.4
作者：
Bokulich NA;Dillon MR;Zhang Y;Rideout JR;Bolyen E;Li H;Albert PS;Caporaso JG
通讯作者：
Caporaso JG