Detection of a group 2 coronavirus in dogs with canine infectious respiratory disease.
Detection of a group 2 coronavirus in dogs with canine infectious respiratory disease.
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DOI：
10.1016/s0042-6822(03)00160-0
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发表时间：
2003-06-05
期刊：
影响因子：
3.7
通讯作者：
Brownlie J
中科院分区：
文献类型：
--
作者：
Erles K;Toomey C;Brooks HW;Brownlie J
对某大型犬舍犬传染性呼吸道疾病的病因进行了调查。从患病犬的呼吸道采集的组织样本使用聚合酶基因的保守引物使用RT-PCR检测冠状病毒的存在。4个阳性样品的序列分析表明，存在的冠状病毒与牛和人冠状病毒（株OC 43）在其聚合酶和刺突基因的高度相似性，而肠道犬冠状病毒中的可比基因的相似性较低。通过RT-PCR在32/119的气管和20/119的肺样本中检测到这种犬呼吸道冠状病毒（CRCV），在具有轻度临床症状的犬中检测到最高的流行率。血清学分析表明，在进入犬舍当天存在CRCV抗体，可降低患呼吸道疾病的风险。
An investigation into the causes of canine infectious respiratory disease was carried out in a large rehoming kennel. Tissue samples taken from the respiratory tract of diseased dogs were tested for the presence of coronaviruses using RT–PCR with conserved primers for the polymerase gene. Sequence analysis of four positive samples showed the presence of a coronavirus with high similarity to both bovine and human coronavirus (strain OC43) in their polymerase and spike genes, whereas there was a low similarity to comparable genes in the enteric canine coronavirus. This canine respiratory coronavirus (CRCV) was detected by RT–PCR in 32/119 tracheal and 20/119 lung samples, with the highest prevalence being detected in dogs with mild clinical symptoms. Serological analysis showed that the presence of antibodies against CRCV on the day of entry into the kennel decreased the risk of developing respiratory disease.
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DOI：
10.3181/00379727-127-32618
发表时间：
1968-01-01
影响因子：
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作者：
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通讯作者：
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影响因子：
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DOI：
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发表时间：
2000-05-15
期刊：
JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
影响因子：
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作者：
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通讯作者：
Loan, RW
影响因子：
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作者：
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