The role of reactive oxygen in the development of Ramularia leaf spot disease in barley seedlings.
The role of reactive oxygen in the development of Ramularia leaf spot disease in barley seedlings.
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DOI：
10.1093/aob/mcx170
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发表时间：
2018-03-05
期刊：
影响因子：
4.2
通讯作者：
Brown JKM
中科院分区：
文献类型：
--
作者：
McGrann GRD;Brown JKM
小白菜拉姆菌（Ramularia colo -cygni）是一种子囊菌真菌，主要作为一种良性内生菌定植大麦，尽管这种相互作用可以成为致病性的，导致拉姆菌叶斑病（RLS）。研究了导致真菌从内生真菌转变为坏死性寄生虫和疾病症状发展的因素，特别是活性氧。人工接种的大麦品种对RLS的部分抗性与接种前暴露于非生物胁迫有关。组织化学和分子化学分析确定了大肠杆菌定殖对活性氧积累和抗氧化基因表达的影响。将抗氧化酶缺陷、氧化还原状态改变或无功能叶绿体的大麦系的RLS发育与真菌生物量积累进行比较，以确定这些因素如何影响疾病症状的表达。暴露于非生物胁迫下会增加所有易感和部分抗性大麦品种的症状发展，与叶片中过氧化氢（H2O2）水平升高有关。在转基因和突变植株中，抗氧化酶超氧化物歧化酶和过氧化氢酶活性的降低对病害转变没有影响，而在真菌无症状生长期间操纵H2O2水平会增加大多数易感品种的病害症状，但对部分抗性植株没有影响。当大麦突变体被cycolo -cygni或白化突变体感染时，绿叶面积迅速损失，表现出RLS症状的发展减少。这些结果表明，在幼苗中，通常内生真菌col -cygni的致病转变不是由衰老本身引起的，而是由导致宿主活性氧变化的因素促进的。大麦品种在这些因素促进RLS病的程度上有所不同。
Ramularia collo-cygni is an ascomycete fungus that colonizes barley primarily as a benign endophyte, although this interaction can become pathogenic, causing the disease Ramularia leaf spot (RLS). Factors, particularly reactive oxygen species, that resulted in the transition of the fungus from endophyte to necrotrophic parasite and the development of disease symptoms were investigated. Disease development in artificially inoculated seedlings of barley varieties varying in partial resistance to RLS was related to exposure to abiotic stress prior to inoculation. Histochemical and molecular analysis determined the effect of R. collo-cygni colonization on accumulation of reactive oxygen species and antioxidant gene expression. Development of RLS on barley lines defective in antioxidant enzymes and with altered redox status or non-functional chloroplasts was compared with the accumulation of fungal biomass to determine how these factors affect disease symptom expression. Exposure to abiotic stress increased symptom development in all susceptible and most partially resistant barley varieties, in association with greater hydrogen peroxide (H2O2) levels in leaves. Decreased activity of the antioxidant enzymes superoxide dismutase and catalase in transgenic and mutant plants had no effect on the disease transition, whereas manipulation of H2O2 levels during asymptomatic growth of the fungus increased disease symptoms in most susceptible varieties but not in partially resistant plants. Barley mutants that undergo rapid loss of green leaf area when infected by R. collo-cygni or albino mutants with non-functional chloroplasts showed reduced development of RLS symptoms. These results imply that in seedlings the pathogenic transition of the normally endophytic fungus R. collo-cygni does not result from senescence as such, but rather is promoted by factors that result in changes to host reactive oxygen species. Barley varieties vary in the extent to which these factors promote RLS disease.
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影响因子：
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