Dynamics of a Dual SARS-CoV-2 Lineage Co-Infection on a Prolonged Viral Shedding COVID-19 Case: Insights into Clinical Severity and Disease Duration.
Dynamics of a Dual SARS-CoV-2 Lineage Co-Infection on a Prolonged Viral Shedding COVID-19 Case: Insights into Clinical Severity and Disease Duration.
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DOI：
10.3390/microorganisms9020300
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发表时间：
2021-02-02
期刊：
影响因子：
4.5
通讯作者：
Tavares M
中科院分区：
文献类型：
--
作者：
Pedro N;Silva CN;Magalhães AC;Cavadas B;Rocha AM;Moreira AC;Gomes MS;Silva D;Sobrinho-Simões J;Ramos A;Cardoso MJ;Filipe R;Palma P;Ceia F;Silva S;Guimarães JT;Sarmento A;Fernandes V;Pereira L;Tavares M
目前，全世界已知少数经分子证实的严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)症状性再感染病例，在48至142天的间隔期后，第一次感染得到解决，随后第二次感染。我们报告了一例临床上严重且旷日持久的病毒脱落性冠状病毒病2019年(新冠肺炎)病例的多成分研究，病例为一名17岁葡萄牙女性。她有两次住院治疗，总共19次RT-PCR检测，大部分为阳性，并在97天结束时从家中隔离出院。对7个系列样本和她感染母亲的诊断样本中的病毒基因组进行了测序。在7个样本上筛选了人类全基因组阵列(>；900K)，并对3个晚期样本的分离物进行了体外培养。这名患者同时感染了两个SARS-CoV-2谱系，这两个谱系属于不同的分支，并由六个变异株分开。在诊断时，20A谱系是绝对的(与患者的母亲共享)，但9天后，20B谱系的频率为3%，两个月后，20B谱系的频率为100%。900K图谱确认了系列样本中患者的身份，并允许我们推断她在葡萄牙人群队列的正态分布范围内具有住院和严重呼吸道疾病的多基因风险分数。早期的动态合并感染可能是导致这名原本健康的年轻患者感染新冠肺炎的严重程度，以及她延长SARS-CoV-2脱落期的原因之一。
A few molecularly proven severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases of symptomatic reinfection are currently known worldwide, with a resolved first infection followed by a second infection after a 48 to 142-day intervening period. We report a multiple-component study of a clinically severe and prolonged viral shedding coronavirus disease 2019 (COVID-19) case in a 17-year-old Portuguese female. She had two hospitalizations, a total of 19 RT-PCR tests, mostly positive, and criteria for releasing from home isolation at the end of 97 days. The viral genome was sequenced in seven serial samples and in the diagnostic sample from her infected mother. A human genome-wide array (>900 K) was screened on the seven samples, and in vitro culture was conducted on isolates from three late samples. The patient had co-infection by two SARS-CoV-2 lineages, which were affiliated in distinct clades and diverging by six variants. The 20A lineage was absolute at the diagnosis (shared with the patient’s mother), but nine days later, the 20B lineage had 3% frequency, and two months later, the 20B lineage had 100% frequency. The 900 K profiles confirmed the identity of the patient in the serial samples, and they allowed us to infer that she had polygenic risk scores for hospitalization and severe respiratory disease within the normal distributions for a Portuguese population cohort. The early-on dynamic co-infection may have contributed to the severity of COVID-19 in this otherwise healthy young patient, and to her prolonged SARS-CoV-2 shedding profile.
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影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
影响因子：
4.6
作者：
Yuan, Bo;Liu, Han-Qing;Song, Shuo
通讯作者：
Song, Shuo
DOI：
10.1016/j.biopha.2021.111272
发表时间：
2021-04
期刊：
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
影响因子：
--
作者：
Dos Santos WG
通讯作者：
Dos Santos WG
影响因子：
14.9
作者：
Letunic, Ivica;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
64.8
作者：
Arvin, Ann M.;Fink, Katja;Virgin, Herbert W.
通讯作者：
Virgin, Herbert W.