GmSALT3, Which Confers Improved Soybean Salt Tolerance in the Field, Increases Leaf Cl(-) Exclusion Prior to Na(+) Exclusion But Does Not Improve Early Vigor under Salinity.
GmSALT3, Which Confers Improved Soybean Salt Tolerance in the Field, Increases Leaf Cl(-) Exclusion Prior to Na(+) Exclusion But Does Not Improve Early Vigor under Salinity.
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DOI：
10.3389/fpls.2016.01485
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发表时间：
2016
影响因子：
5.6
通讯作者：
Guan R
中科院分区：
文献类型：
--
作者：
Liu Y;Yu L;Qu Y;Chen J;Liu X;Hong H;Liu Z;Chang R;Gilliham M;Qiu L;Guan R
土壤盐分降低大豆生长和产量。最近发现的GmSALT 3（3号染色体上的大豆耐盐相关基因）具有提高盐渍条件下大豆产量的潜力。在这里，我们评估了GmSALT 3对大豆在盐或非盐条件下的性能的影响。以盐敏感品种85-140（S）和耐盐品种铁丰8号（T）为材料，通过分子标记辅助选择，获得了3组近等基因系（NIL-T和NIL-S），每对NIL-T和NIL-S之间的遗传相似性为95.6-99.3%。每个NIL-T; 782-T，820-T和860-T，在3号染色体上含有一个共同的~1000 kb片段，其中GmSALT 3位于该片段上。我们表明，GmSALT 3不有助于盐胁迫下的出苗率或早期活力的改善。在NaCl胁迫下，NIL-T系叶片Na+积累量显著低于NIL-S系，而K+积累量在NIL-T系和NIL-S系间差异不显著;不同遗传背景对NIL-T系叶片Na+积累量的影响不同。此外，NIL-T线积累较少的Cl-在叶片和更多的根之前，任何差异的Na+，在现场，他们积累较少的荚壁Cl-比相应的NIL-S线。在非盐碱地条件下，未观察到每对NIL-T和NIL-S系内产量相关性状的显著差异，表明具有GmSALT 3基因不存在产量惩罚。相比之下，在盐碱地条件下，NIL-T系具有比相应的NIL-S系显著更大的植物种子重量和百粒重，这意味着GmSALT 3赋予盐碱地中的大豆植物产量优势。我们的研究结果表明，GmSALT 3介导的调节Na+和Cl-在大豆中的积累，并有助于提高大豆产量，通过保持较高的种子重量在盐胁迫下。
Soil salinity reduces soybean growth and yield. The recently identified GmSALT3 (Glycine max salt Tolerance-associated gene on chromosome 3) has the potential to improve soybean yields in salinized conditions. Here we evaluate the impact of GmSALT3 on soybean performance under saline or non-saline conditions. Three sets of near isogenic lines (NILs), with genetic similarity of 95.6–99.3% between each pair of NIL-T and NIL-S, were generated from a cross between two varieties 85–140 (salt-sensitive, S) and Tiefeng 8 (salt-tolerant, T) by using marker-assisted selection. Each NIL-T; 782-T, 820-T and 860-T, contained a common ~1000 kb fragment on chromosome 3 where GmSALT3 was located. We show that GmSALT3 does not contribute to an improvement in seedling emergence rate or early vigor under salt stress. However, when 12-day-old seedlings were exposed to NaCl stress, the NIL-T lines accumulated significantly less leaf Na+ compared with their corresponding NIL-S, while no significant difference of K+ concentration was observed between NIL-T and NIL-S; the magnitude of Na+ accumulation within each NIL-T set was influenced by the different genetic backgrounds. In addition, NIL-T lines accumulated less Cl- in the leaf and more in the root prior to any difference in Na+; in the field they accumulated less pod wall Cl- than the corresponding NIL-S lines. Under non-saline field conditions, no significant differences were observed for yield related traits within each pair of NIL-T and NIL-S lines, indicating there was no yield penalty for having the GmSALT3 gene. In contrast, under saline field conditions the NIL-T lines had significantly greater plant seed weight and 100-seed weight than the corresponding NIL-S lines, meaning GmSALT3 conferred a yield advantage to soybean plants in salinized fields. Our results indicated that GmSALT3 mediated regulation of both Na+ and Cl- accumulation in soybean, and contributes to improved soybean yield through maintaining a higher seed weight under saline stress.
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