Ovary Abortion Induced by Combined Waterlogging and Shading Stress at the Flowering Stage Involves Amino Acids and Flavonoid Metabolism in Maize.
Ovary Abortion Induced by Combined Waterlogging and Shading Stress at the Flowering Stage Involves Amino Acids and Flavonoid Metabolism in Maize.
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开花期涝渍和遮荫胁迫联合引起的卵巢败育涉及玉米的氨基酸和类黄酮代谢
DOI：
10.3389/fpls.2021.778717
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发表时间：
2021
影响因子：
5.6
通讯作者：
Li C
中科院分区：
文献类型：
--
作者：
Zhou J;Tian L;Wang S;Li H;Zhao Y;Zhang M;Wang X;An P;Li C
玉米（Zea mays L.）华北平原的农作物在开花期经常遭遇阴雨连绵。这会同时导致水涝和遮荫胁迫，并导致巨大的产量损失，但这些产量损失的原因在很大程度上仍然未知。为了探讨导致玉米开花期受淹水和遮光胁迫的影响而导致产量损失的因素，我们对淹水、遮光和淹水和遮光（WS）处理的玉米植株进行了表型、生理和准靶向代谢组学分析。表型和生理指标分析表明，与单独涝渍或遮荫相比，WS导致源强度降低，对卵巢和穗尖花丝生长的抑制更严重，吐丝数减少，卵巢败育率更高。碳含量和酶活性的变化不能解释卵巢败育。代谢组学分析表明，卵巢和花丝中发生的事件与败育密切相关，WS迫使卵巢将更多的资源分配给参与应激反应的氨基酸合成，抑制能量代谢、谷胱甘肽代谢和蛋氨酸补救途径，并过度积累H2O2。在蚕丝中，WS导致具有抗氧化能力的特定类黄酮代谢物的积累水平降低，并导致H2O2的过度积累。因此，与各单一胁迫相比，WS更严重地干扰了正常的代谢过程，导致卵巢和花丝更严重的氧化应激。卵巢中参与应激反应的氨基酸和花丝中具有抗氧化能力的类黄酮代谢产物在卵巢败育过程中起重要作用。这些结果确定了育种计划中选择的新性状和基因组编辑的目标，以增加WS胁迫下的玉米产量。
Maize (Zea mays L.) crops on the North China Plain are often subject to continuous overcast rain at the flowering stage. This causes waterlogging and shading stresses simultaneously and leads to huge yield losses, but the causes of these yield losses remain largely unknown. To explore the factors contributing to yield loss caused by combined waterlogging and shading stress at the flowering stage, we performed phenotypic, physiological, and quasi-targeted metabolomics analyses of maize plants subjected to waterlogging, shading, and combined waterlogging and shading (WS) treatments. Analyses of phenotypic and physiological indexes showed that, compared with waterlogging or shading alone, WS resulted in lower source strength, more severe inhibition of ovary and silk growth at the ear tip, a reduced number of emerged silks, and a higher rate of ovary abortion. Changes in carbon content and enzyme activity could not explain the ovary abortion in our study. Metabolomic analyses showed that the events occurred in ovaries and silks were closely related to abortion, WS forced the ovary to allocate more resources to the synthesis of amino acids involved in the stress response, inhibited the energy metabolism, glutathione metabolism and methionine salvage pathway, and overaccumulation of H2O2. In silks, WS led to lower accumulation levels of specific flavonoid metabolites with antioxidant capacity, and to over accumulation of H2O2. Thus, compared with each single stress, WS more seriously disrupted the normal metabolic process, and resulted more serious oxidative stress in ovaries and silks. Amino acids involved in the stress response in ovaries and specific flavonoid metabolites with antioxidant capacity in silks play important roles during ovary abortion. These results identify novel traits for selection in breeding programs and targets for genome editing to increase maize yield under WS stress.
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