Gene-expression profile and postpartum transition of bovine endometrial side population cells†
Gene-expression profile and postpartum transition of bovine endometrial side population cells†
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牛子宫内膜侧群细胞的基因表达谱和产后转变†
DOI：
10.1093/biolre/ioab004
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发表时间：
2021
影响因子：
3.6
通讯作者：
Matsuyama Shuichi
中科院分区：
文献类型：
--
作者：
Tatebayashi Ryoki;Nakamura Sho;Minabe Shiori;Furusawa Tadashi;Abe Ryoya;Kajisa Miki;Morita Yasuhiro;Ohkura Satoshi;Kimura Koji;Matsuyama Shuichi
牛产后子宫内膜再生的机制尚不清楚。在这里，我们假设牛子宫内膜干细胞/祖细胞参与了产后子宫内膜的再生。流式细胞术分析发现子宫内膜间质细胞中存在侧群（SP）细胞。子宫内膜SP细胞分化为成骨细胞和脂肪细胞。RNA-seq数据显示，该基因在牛子宫内膜SP细胞和主要群体细胞中的表达模式不同。基因集富集分析鉴定了SP细胞中干细胞性基因的富集。SP细胞中显著上调的基因包含多个干细胞标记基因（错误发现率< 0.01）。对SP细胞中上调基因的基因本体（GO）分析显示，与RNA代谢过程和转录相关的术语富集。京都基因与基因组百科（KEGG）通路分析显示，SP细胞中上调基因的信号通路富集，与干细胞/祖细胞的维持和分化相关。在SP细胞下调基因的GO和KEGG通路分析中，涉及TCA循环的术语丰富。这些结果支持了牛子宫内膜SP细胞具有体细胞干/祖细胞特征的假设。SP细胞与子宫内膜细胞的比例在分娩后第9 ~ 11天最低，此后逐渐升高。SP细胞可分化为上皮细胞。综上所述，这些结果表明，牛子宫内膜SP细胞在产犊后立即暂时减少，可能是由于它们分化为新的子宫内膜细胞。
The mechanism of bovine endometrial regeneration after parturition remains unclear. Here, we hypothesized that bovine endometrial stem/progenitor cells participate in the postpartum regeneration of the endometrium. Flow cytometry analysis identified the presence of side population (SP) cells among endometrial stromal cells. Endometrial SP cells were shown to differentiate into osteoblasts and adipocytes. RNA-seq data showed that the gene expression pattern was different between bovine endometrial SP cells and main population cells. Gene Set Enrichment Analysis identified the enrichment of stemness genes in SP cells. Significantly (false discovery rate < 0.01) upregulated genes in SP cells contained several stem cell marker genes. Gene ontology (GO) analysis of the upregulated genes in SP cells showed enrichment of terms related to RNA metabolic process and transcription. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of upregulated genes in SP cells revealed enrichment of signaling pathways associated with maintenance and differentiation of stem/progenitor cells. The terms involved in TCA cycles were enriched in GO and KEGG pathway analysis of downregulated genes in SP cells. These results support the assumption that bovine endometrial SP cells exhibit characteristics of somatic stem/progenitor cells. The ratio of SP cells to endometrial cells was lowest on days 9–11 after parturition, which gradually increased thereafter. SP cells were shown to differentiate into epithelial cells. Collectively, these results suggest that bovine endometrial SP cells were temporarily reduced immediately after calving possibly due to their differentiation to provide new endometrial cells.
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影响因子：
7.2
作者：
Grafe I;Alexander S;Peterson JR;Snider TN;Levi B;Lee B;Mishina Y
通讯作者：
Mishina Y
影响因子：
6.1
作者：
Kato, Kiyoko;Yoshimoto, Momoko;Wake, Norio
通讯作者：
Wake, Norio
影响因子：
3.7
作者：
Huang CC;Orvis GD;Wang Y;Behringer RR
通讯作者：
Behringer RR
影响因子：
6.7
作者：
S. Tsuji;M. Yoshimoto;Kentaro Takahashi;Y. Noda;T. Nakahata;T. Heike
通讯作者：
S. Tsuji;M. Yoshimoto;Kentaro Takahashi;Y. Noda;T. Nakahata;T. Heike
影响因子：
23.9
作者：
Ryall JG;Cliff T;Dalton S;Sartorelli V
通讯作者：
Sartorelli V