A Mini-Review on Detection Methods of Microcystins.
A Mini-Review on Detection Methods of Microcystins.
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DOI：
10.3390/toxins12100641
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发表时间：
2020-10-04
期刊：
影响因子：
4.2
通讯作者：
Yang F
中科院分区：
文献类型：
--
作者：
Massey IY;Wu P;Wei J;Luo J;Ding P;Wei H;Yang F
蓝藻水华(CyanoHabs)产生的微囊藻毒素(MC)与动物和人类的肝脏毒性有关。MC的变种有270多种。由于MCS的毒性后果，人们一直在对其进行研究。监测水质以评估MC的存在是极其重要的，尽管这往往很困难，因为氰化氢化合物可能会产生多种MC变异体，而且它们在水中的浓度很低。为了有效地管理和控制这些毒素，防止它们的健康风险，需要能够灵敏、快速、可靠地检测MCs的方法。本文综述了检测MCs的三种主要分析方法，包括生物(小鼠生物检测)、生化(蛋白磷酸酶抑制检测和酶联免疫吸附检测)和化学检测(高效液相色谱、液-质联用、高效毛细管电泳法、气相色谱)，以及新兴的生物传感器方法。此外，还介绍了这些方法的新发展和应用现状，以及它们的优点和局限性。最后，本文还对方法的应用和改进提出了建议和未来的研究方向。
Cyanobacterial harmful algal blooms (CyanoHABs) produce microcystins (MCs) which are associated with animal and human hepatotoxicity. Over 270 variants of MC exist. MCs have been continually studied due of their toxic consequences. Monitoring water quality to assess the presence of MCs is of utmost importance although it is often difficult because CyanoHABs may generate multiple MC variants, and their low concentration in water. To effectively manage and control these toxins and prevent their health risks, sensitive, fast, and reliable methods capable of detecting MCs are required. This paper aims to review the three main analytical methods used to detect MCs ranging from biological (mouse bioassay), biochemical (protein phosphatase inhibition assay and enzyme linked immunosorbent assay), and chemical (high performance liquid chromatography, liquid chromatography-mass spectrometry, high performance capillary electrophoresis, and gas chromatography), as well as the newly emerging biosensor methods. In addition, the current state of these methods regarding their novel development and usage, as well as merits and limitations are presented. Finally, this paper also provides recommendations and future research directions towards method application and improvement.
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作者：
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影响因子：
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通讯作者：
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