Substantial alterations of the cutaneous bacterial biota in psoriatic lesions.
Substantial alterations of the cutaneous bacterial biota in psoriatic lesions.
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DOI：
10.1371/journal.pone.0002719
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发表时间：
2008-07-23
期刊：
影响因子：
3.7
通讯作者：
Blaser MJ
中科院分区：
文献类型：
--
作者：
Gao Z;Tseng CH;Strober BE;Pei Z;Blaser MJ
对于牛皮癣，一种皮肤的特发性炎症性疾病，微生物群尚未定义使用培养独立的方法。我们采用宽范围的16S rDNA PCR检测古细菌和细菌，检测正常皮肤和银屑病皮肤的微生物群。从6例患者中获得19份皮肤样本（病变皮肤13份，正常皮肤6份）。对提取的DNA进行大范围PCR，并将1925个克隆产物与先前报道的健康人的2038个克隆产物进行比较。以98%的序列同源性作为种界，1841个（95.6%）克隆与已知细菌16S rDNA相似，代表6门、86属或189种水平的操作分类单位（SLOTU）；同源性<98%的无性系84个（4.4%）可能是新种。银屑病皮损中最丰富和最多样化的门是厚壁菌门（46.2%），与未受累患者（39.0%）和健康人（24.4%）的皮肤样本相比，显著（P<0.001）过度代表。相比之下，放线菌是健康人（47.6%）和患者（47.8%）正常皮肤样本中最常见和最多样化的门，但在银屑病病变样本（37.3%）中，放线菌的代表性明显不足（P<0.01）。银屑病皮损中丙酸杆菌种类的代表性（2.9±5.5%）低于正常人（21.1±18.2%，P<0.001），而银屑病患者正常皮肤中丙酸杆菌种类的代表性为中等水平（12.3±21.6%）。我们的结论是，牛皮癣与皮肤细菌生物群组成和表现的实质性改变有关。
For psoriasis, an idiopathic inflammatory disorder of the skin, the microbial biota has not been defined using cultivation-independent methods. We used broad-range 16S rDNA PCR for archaea and bacteria to examine the microbiota of normal and psoriatic skin. From 6 patients, 19 cutaneous samples (13 from diseased skin and 6 from normal skin) were obtained. Extracted DNA was subjected to the broad range PCR, and 1,925 cloned products were compared with 2,038 products previously reported from healthy persons. Using 98% sequence identity as a species boundary, 1,841 (95.6%) clones were similar to known bacterial 16S rDNA, representing 6 phyla, 86 genera, or 189 species-level operational taxonomic unit (SLOTU); 84 (4.4%) clones with <98% identity probably represented novel species. The most abundant and diverse phylum populating the psoriatic lesions was Firmicutes (46.2%), significantly (P<0.001) overrepresented, compared to the samples from uninvolved skin of the patients (39.0%) and healthy persons (24.4%). In contrast, Actinobacteria, the most prevalent and diverse phylum in normal skin samples from both healthy persons (47.6%) and the patients (47.8%), was significantly (P<0.01) underrepresented in the psoriatic lesion samples (37.3%). Representation of Propionibacterium species were lower in the psoriatic lesions (2.9±5.5%) than from normal persons (21.1±18.2%; P<0.001), whereas normal skin from the psoriatic patients showed intermediate levels (12.3±21.6%). We conclude that psoriasis is associated with substantial alteration in the composition and representation of the cutaneous bacterial biota.
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影响因子：
120.7
作者：
Gelfand, Joel M.;Neimann, Andrea L.;Troxel, Andrea B.
通讯作者：
Troxel, Andrea B.
影响因子：
5.8
作者：
Huber, T;Faulkner, G;Hugenholtz, P
通讯作者：
Hugenholtz, P
DOI：
10.1073/pnas.0308766101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Lepp, PW;Brinig, MM;Relman, DA
通讯作者：
Relman, DA
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
影响因子：
9.4
作者：
Paulino, Luciana C.;Tseng, Chi-Hong;Blaser, Martin J.
通讯作者：
Blaser, Martin J.