The effect of fresh bone marrow cells on reconstruction of mouse calvarial defect combined with calvarial osteoprogenitor cells and collagen-apatite scaffold.
The effect of fresh bone marrow cells on reconstruction of mouse calvarial defect combined with calvarial osteoprogenitor cells and collagen-apatite scaffold.
复制标题
新鲜骨髓细胞对小鼠钙钙缺陷的重建以及钙化骨化剂细胞和胶原蛋白磷灰石支架的影响。
DOI：
10.1002/term.1490
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发表时间：
2013-12
影响因子：
3.3
通讯作者：
Wei, Mei
中科院分区：
文献类型：
--
作者：
Yu, Xiaohua;Wang, Liping;Peng, Fei;Jiang, Xi;Xia, Zengmin;Huang, Jianping;Rowe, David;Wei, Mei
关键词：
新鲜骨髓细胞已经显示出其作为成骨供体细胞的优势，但新鲜骨髓细胞与用于骨愈合的其他供体细胞之间的组合尚未得到充分探索。为了突出新鲜骨髓细胞对基于支架的骨再生的影响，将颅骨骨祖细胞（OPC）和骨髓细胞（BMC）单独或组合用作与胶原/磷灰石支架组合用于颅骨缺损愈合的供体细胞。使用组织学和GFP成像分析评估宿主和供体对骨形成的贡献。虽然不同来源的细胞形成的新骨量无显著差异，但骨缺损形成的来源主要取决于所用的供体细胞类型：当仅使用颅骨OPC作为供体细胞时，观察到供体来源的骨愈合而不是宿主来源的骨长入;当仅负载新鲜BMC时，宿主骨可沿支架的层状结构沿着向缺损处生长，但新骨形成量明显低于仅负载颅骨OPC的缺损处。颅骨OPC和新鲜BMC的组合具有与单独加载颅骨骨祖细胞的组相似的新骨形成量，但不诱导任何宿主来源的骨形成。这些结果为新鲜BMC在骨组织工程中诱导宿主-植入物整合的重要性提供了令人信服的证据。
Fresh bone marrow cells have already exhibited its advantages as osteogenic donor cells, but the combination between fresh bone marrow cells and other donor cells utilized for bone healing has not been fully explored. To highlight the impact of fresh bone marrow cells on scaffold-based bone regeneration, single or a combination of calvarial osteoprogenitor cells (OPC) and bone marrow cells (BMC) were used as donor cells combined with collagen/apatite scaffold for calravieal defect healing. The host and donor contributions to bone formation were assessed using histological and GFP imaging analysis. Although the amount of new bone formed by different cell sources did not show significant differences, the origin of the bone formation in the defects mainly depended on the types of donor cells employed: when only calvarial OPC were used as donor cells, a donor-derived bone healing instead of host-derived bone ingrowth was observed; when only fresh BMC were loaded, the host bone could grow into the defect along the lamellar structure of the scaffolds, but the amount of new bone formed was significantly lower than the defect loaded with calvarial OPC only. The combination of calvarial OPC and fresh BMC had similar amount of new bone formation as the group loaded with calvarial osteoprogenitors alone, but did not induce any host-derived bone formation. These results provide compelling evidence of the importance of fresh BMC to induce host-implant integration in bone tissue engineering.
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影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP
影响因子：
4.9
作者：
Jegoux, Franck;Goyenvalle, Eric;Aguado, Eric
通讯作者：
Aguado, Eric
DOI：
10.3109/17453678909149289
发表时间：
1989-06-01
期刊：
ACTA ORTHOPAEDICA SCANDINAVICA
影响因子：
--
作者：
OHGUSHI, H;GOLDBERG, VM;CAPLAN, AI
通讯作者：
CAPLAN, AI
DOI：
10.1002/jbm.b.30889
发表时间：
2008-02-01
影响因子：
3.4
作者：
Qu, Haibo;Wei, Mei
通讯作者：
Wei, Mei
影响因子：
4.9
作者：
Dumas, Aline;Moreau, Marie-Francoise;Chappard, Daniel
通讯作者：
Chappard, Daniel