An integrated transcriptomic and proteomic analysis reveals toxin arsenal of a novel Antarctic jellyfish Cyanea sp.
An integrated transcriptomic and proteomic analysis reveals toxin arsenal of a novel Antarctic jellyfish Cyanea sp.
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综合转录组和蛋白质组分析揭示了一种新型南极水母 Cyanea sp 的毒素库。
DOI：
10.1016/j.jprot.2019.103483
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发表时间：
2019-09
影响因子：
3.3
通讯作者：
Xiao Liang
中科院分区：
文献类型：
--
作者：
Liang Hongyu;Jiang Guixian;Wang Tengjiao;Zhang Jie;Liu Wenwen;Xu Zheng;Zhang Jing;Xiao Liang
水母是海洋中常见的有毒浮游动物。我们在南极洲水下成功捕获了一种水母200 m，并通过形态学检查和MT-CO1系统发育分析将其鉴定为水母cyanea sp.。通过转录组测序共收获了40,468个单基因。我们还成功地用NR数据库注释了12,955（32.01%）个unique，用SWISSPROT数据库注释了10,882（26.89%）个unique，用GO数据库注释了4951（12.23%）个unique，用KEGG数据库注释了4901（12.11%）个unique。在蛋白质组学分析中，利用构建的转录组作为数据库，共收获了11159个肽和2630个蛋白。在GO和KEGG数据库中分别有771个（29.31%）和841个（31.98%）蛋白被注释。此外，从145个毒素相关单基因中成功筛选出29个与之匹配的毒性蛋白，包括6个金属蛋白酶、4个磷脂酶、2个丝氨酸蛋白酶、1个丝氨酸蛋白酶抑制剂、7个毒素相关毒液和9个其他毒素。本研究首次利用转录组学和蛋白质组学方法鉴定了极地水母cyanea sp.，这些数据可以进一步为鉴定可能在寒冷环境中发挥作用的极地水母衍生先导化合物提供公共数据库。随着对海洋生物多样性和全球变暖的讨论越来越多，极地物种逐渐成为研究的焦点。据我们所知，在pubMed上只有一篇关于南极潜行水母（Haliclystus antarcticus Pfeffer）线粒体基因组的论文。在本次研究中，我们于2016年底乘坐科学考察船“雪龙”号在南大洋（南纬60°29′57”，西经52°11′44”）捕获了一种水母，命名为BD-4。虽然样品在- 20 °C的条件下在船上储存和运输了两个多月，但我们成功地提取了总RNA，并进行了分子物种鉴定和新转录组学和蛋白质组学的联合分析。除了传统的生物信息学技术，如GO和KEGG注释，我们筛选和列出有毒蛋白质，排列序列，模拟三维结构，并对典型成分进行分子系统发育分析，包括金属蛋白酶和丝氨酸蛋白酶。本研究首次利用新转录组学和蛋白质组学方法鉴定极性水母cyanea sp.，这些数据可以进一步为鉴定极性水母衍生的潜在先导化合物提供公共数据库。
Jellyfish is a common toxic zooplankton in ocean. We successfully captured a kind of jellyfish 200 m underwater in Antarctica, and identified it as a jellyfishCyanea sp.through morphological examination and MT-CO1 phylogenetic analysis. A total of 40,468 unigenes were harvested through transcriptome sequencing. We also successfully annotated 12,955 (32.01%) unigenes with the NR database, 10,882 (26.89%) unigenes with the SWISSPROT database, 4951 (12.23%) unigenes with the GO database, and 4901 (12.11%) unigenes with the KEGG database. In the proteomic analysis, a total of 11,159 peptides and 2630 proteins were harvested using the constructed transcriptome as the database. A number of 771 (29.31%) and 841 (31.98%) proteins were annotated against the GO and KEGG database, respectively. Moreover, a number of 29 toxic proteins matched from the 145 toxin-related unigenes were successfully screened, including 6 metalloproteinases, 4 phospholipases, 2 serine proteases, 1 serine protease inhibitor, 7 toxin-related venom and 9 other toxins. Our study is the first to identify a polar jellyfishCyanea sp.with transcriptomics and proteomics, and these data can further serve as a public database for the identification of potential polar jellyfish-derived lead compounds feasibly functioning in the cold environment.SignificanceWith increasing discussions on marine biodiversity and global warming, polar species have gradually become a focus for research. To the best of our knowledge, there is only one paper in pubMed about the mitochondrial genome of the Antarctic stalked jellyfish Haliclystus antarcticus Pfeffer. In this study, we captured a type of jellyfish (named BD-4) from the Southern Ocean (60°29′57” S, 52°11′44”W) on the scientific expedition ship “Xue Long” at the end of 2016. Although the samples were stored and transported by the ship at only −20 °C for more than two month, we successfully extracted the total RNA, and performed molecular species identification and combined analyses ofde novotranscriptomics and proteomics. In addition to conventional bioinformatics techniques such as GO and KEGG annotation, we screened and listed toxic proteins, aligned the sequences, simulated three-dimensional structures and performed molecular phylogenetic analysis for typical components, including metalloproteinase and serine proteinase. Our study is the first to identify a polar jellyfishCyanea sp.withde novotranscriptomics and proteomics, and these data can further serve as a public database for the identification of potential polar jellyfish-derived lead compounds.
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