Quercetin Alleviates Lipopolysaccharide-Induced Inflammatory Response in Bovine Mammary Epithelial Cells by Suppressing TLR4/NF-κB Signaling Pathway.
Quercetin Alleviates Lipopolysaccharide-Induced Inflammatory Response in Bovine Mammary Epithelial Cells by Suppressing TLR4/NF-κB Signaling Pathway.
复制标题
DOI：
10.3389/fvets.2022.915726
复制
发表时间：
2022
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
作者：
牛乳腺炎是奶牛最常见的临床疾病之一，给奶牛业造成巨大的经济损失。槲皮素是一种重要的黄酮类化合物，存在于多种食品资源中，作为潜在的抗炎和抗氧化剂而受到广泛关注。然而，槲皮素对脂多糖（LPS）诱导的牛乳腺上皮细胞（BMEC）炎症反应和氧化应激的分子机制仍不清楚。本研究的目的是探讨槲皮素对 LPS 诱导的 BMEC 炎症反应、氧化应激和屏障功能的影响。我们的结果表明，与对照组相比，50 和 100 μg/ml 槲皮素和 1 μg/ml LPS 处理对 BMEC 活力没有影响。氧化应激指标及屏障功能相关基因结果表明，100μg/ml槲皮素可有效保护BMEC免受1μg/ml LPS引起的氧化和屏障损伤。此外，与单独的 LPS 相比，在含有 100 μg/ml 槲皮素的 LPS 处理的牛视网膜内皮细胞 (BREC) 中，促炎细胞因子 TNF-α、IL-1β、IL-6 和趋化因子 CXCL2、CXCL5、CCL5 和 CXCL8 的信使 RNA (mRNA) 表达显着降低。更重要的是，LPS处理的BMEC中添加槲皮素后，参与TLR4信号通路的Toll样受体4（TLR4）、CD14、髓样分化蛋白2（MD2）和髓样分化初级反应蛋白（MyD88）基因的mRNA表达显着减弱，表明槲皮素可以抑制TLR4信号通路。此外，免疫细胞荧光显示槲皮素显着抑制LPS诱导的BMEC中NF-κB p65的核转位。因此，槲皮素对LPS诱导的BMEC炎症反应的保护作用可能是由于其抑制TLR4介导的NF-κB信号通路的能力。这些发现表明槲皮素可以作为抗炎试剂来治疗由外源性或内源性LPS释放引起的乳腺炎。
Bovine mastitis is one of the most common clinical diseases in dairy cows, causing huge economic losses to the dairy industry. Quercetin is an important flavonoid existing in many food resources, which has attracted widespread attention as a potential anti-inflammatory and antioxidant. However, the molecular mechanism of quercetin on inflammatory responses and oxidative stress in bovine mammary epithelial cells (BMECs) induced by lipopolysaccharide (LPS) remains unknown. The objective of this study was to investigate the effects of quercetin on inflammation responses, oxidative stress, and barrier function of BMEC induced by LPS. Our results showed that BMEC viability was not affected by treatment with 50 and 100 μg/ml of quercetin and 1 μg/ml of LPS compared with control group. The results of oxidative stress indicators and related genes of barrier function indicated that 100 μg/ml of quercetin effectively protected the BMECs from damage of oxidative and barrier induced by 1 μg/ml of LPS. Moreover, the messenger RNA (mRNA) expressions of pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and chemokines CXCL2, CXCL5, CCL5, and CXCL8 were markedly decreased in the LPS-treated bovine retinal endothelial cells (BRECs) with 100 μg/ml of quercetin relatively to LPS alone. More importantly, the mRNA expressions of toll-like receptor 4 (TLR4), CD14, myeloid differential protein-2 (MD2), and myeloid differentiation primary response protein (MyD88) genes involved in TLR4 signal pathway were significantly attenuated by the addition of quercetin in LPS-treated BMEC, suggesting that quercetin can inhibit the TLR4 signal pathway. In addition, immunocytofluorescence showed that quercetin significantly inhibited the nuclear translocation of NF-κB p65 in BMEC induced by LPS. Therefore, the protective effects of quercetin on inflammatory responses in LPS-induced BMEC may be due to its ability to suppress the TLR4-mediated NF-κB signaling pathway. These findings suggest that quercetin can be used as an anti-inflammatory reagent to treat mastitis induced by exogenous or endogenous LPS release.
登录
查看更多内容
影响因子：
5.9
作者：
Li Y;Yao J;Han C;Yang J;Chaudhry MT;Wang S;Liu H;Yin Y
通讯作者：
Yin Y
影响因子：
30.5
作者：
通讯作者：
--
DOI：
10.1158/1078-0432.ccr-10-1565
发表时间：
2010-12-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Cheng S;Gao N;Zhang Z;Chen G;Budhraja A;Ke Z;Son YO;Wang X;Luo J;Shi X
通讯作者：
Shi X
影响因子：
11
作者：
Citronberg, Jessica S.;Curtis, Keith R.;Hullar, Meredith A. J.
通讯作者：
Hullar, Meredith A. J.
影响因子：
50.5
作者：
Ekstrom, A. M.;Serafini, M.;Bellocco, R.
通讯作者：
Bellocco, R.