Reversible regulation of stem cell niche size associated with dietary control of Notch signalling.
Reversible regulation of stem cell niche size associated with dietary control of Notch signalling.
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DOI：
10.1186/s12861-015-0059-8
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发表时间：
2015-01-31
影响因子：
--
通讯作者：
Baron M
中科院分区：
文献类型：
--
作者：
Bonfini A;Wilkin MB;Baron M
干细胞可以对环境和生理输入作出反应，自适应地重塑组织。干细胞壁龛是否也有类似的反应尚不清楚。果蝇卵巢种系干细胞（GSC）生态位是一个被广泛研究的模型，它由帽细胞组成，帽细胞为GSC维持卵子发生提供锚定和维持信号。先前的研究已经表明饮食和卵子发生的调节之间有很强的联系，这使其成为一个有用的模型系统，用于研究生态位及其相关干细胞的饮食调节。我们发现果蝇卵巢GSC帽细胞生态位是一个动态结构，可以在波动的饮食条件下收缩和扩张。当成蝇转移到营养不良的饮食中时，帽细胞会丢失，当果蝇返回到营养丰富的培养基中时，帽细胞会恢复。帽状细胞和护卫队细胞中的Notch信号同样会随着饮食转向营养贫乏和富营养培养基而减少和恢复。在老年果蝇中，Notch信号的减少与生态位对饮食变化的稳健性下降有关。我们使用Notch温度敏感等位基因证明，Notch信号的去除和恢复也会导致生态位的减少和重新扩展。生态位大小的变化与细胞凋亡或细胞分裂无关。我们发现Notch在成虫胚乳中有两个不同的作用。Notch可以在帽细胞中起作用，防止它们的丢失，而在侧翼护航细胞中激活Notch会导致生态位的扩大。我们提供的证据表明，饮食变化本身就足以改变Notch信号，并可逆地改变野生型成年果蝇的生态位大小。我们发现Notch在不同的细胞中发挥作用来维持和重新扩大生态位，并提出了一个模型，其中细胞命运在帽细胞和侧翼体细胞之间的转变解释了生态位动力学。这些发现揭示了GSC生态位的一种意想不到的可逆可塑性，其反应提供了苍蝇受饮食和年龄调节的生理状态的综合解读。本文的在线版本（doi:10.1186/s12861-015-0059-8）包含补充材料，可供授权用户使用。
Stem cells can respond to environmental and physiological inputs to adaptively remodel tissues. Little is known about whether stem cell niches are similarly responsive. The Drosophila ovary germline stem cell (GSC) niche is a well-studied model, which is comprised of cap cells that provide anchorage and maintenance signals for GSCs to maintain oogenesis. Previous studies have shown a strong link between diet and the regulation of oogenesis, making this a useful model system in which to investigate dietary regulation of the niche and its associated stem cells. We show that the Drosophila ovary GSC cap cell niche is a dynamic structure, which can contract and expand in fluctuating dietary conditions. Cap cells are lost when adult flies are shifted to nutrient poor diet and are restored after returning flies to nutrient-rich medium. Notch signalling in cap and escort cells is similarly reduced and restored by dietary shifts to nutrient poor and rich media. In old flies decreased Notch signalling is associated with decreased robustness of the niche to dietary changes. We demonstrated using a Notch temperature sensitive allele that removal and restoration of Notch signalling also leads to a reduction and re-expansion of the niche. Changes in niche size were not associated with apoptosis or cell division. We identified two distinct roles for Notch in the adult germarium. Notch can act in cap cells to prevent their loss while activation of Notch in the flanking escort cells results in expansion of the niche. We provide evidence that dietary changes alone are sufficient to alter Notch signalling and reversibly change niche size in the adult in wild type flies. We show Notch acts in different cells to maintain and re-expand the niche and propose a model in which cell fate transitions between cap cells and flanking somatic cells accounts for niche dynamics. These findings reveal an unexpected reversible plasticity of the GSC niche whose responses provide an integrated read out of the physiological status of the fly that is modulated by diet and age. The online version of this article (doi:10.1186/s12861-015-0059-8) contains supplementary material, which is available to authorized users.
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影响因子：
4.6
作者：
DiNardo, Stephen;Okegbe, Tishina;Terry, Natalie
通讯作者：
Terry, Natalie
影响因子：
64.5
作者：
Kuo, Chay T.;Mirzadeh, Zaman;Jan, Yuh-Nung
通讯作者：
Jan, Yuh-Nung
DOI：
10.1073/pnas.0809144106
发表时间：
2009-01-27
影响因子：
11.1
作者：
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通讯作者：
Drummond-Barbosa, Daniela
影响因子：
64.8
作者：
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通讯作者：
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影响因子：
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作者：
Leeman, Kristen T.;Fillmore, Christine M.;Kim, Carla F.
通讯作者：
Kim, Carla F.