Establishment of an experimental glaucoma animal model: A comparison of microbead injection with or without hydroxypropyl methylcellulose.
Establishment of an experimental glaucoma animal model: A comparison of microbead injection with or without hydroxypropyl methylcellulose.
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DOI：
10.3892/etm.2017.4728
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发表时间：
2017-09
影响因子：
2.7
通讯作者：
Ding C
中科院分区：
文献类型：
--
作者：
Liu H;Ding C
为了建立青光眼的实验动物模型，本研究比较了羟丙基甲基纤维素（HPM）与不羟丙基甲基纤维素（HPM）微珠注射液的作用。在视网膜神经节细胞（RGCs）中Thy1启动子控制下，以表达青色荧光蛋白（CFP）的C57BL/6小鼠和转基因小鼠为实验对象建立该模型。将12 ~ 20周龄的C57BL/6小鼠随机分为三组，分别在眼前房进行不同程度的注射。A组（微珠组）给予微珠2µl （10×106 beads/ml）和空气1µl。B组（微珠+ HPM）给予微珠2µl， HPM 1µl。C组（对照组）给予PBS 2µl，空气1µl。在表面麻醉下，每天用眼压计测量眼内压（IOP），持续1个月。与对照组相比，单次注射微珠，无论有无HPM，都能引起一致的IOP升高。在Thy1-CFP小鼠的前房眼内注射2µl微珠和1µl HPM，随后通过共聚焦扫描激光显微镜观察体内同一视网膜区域CFP+ RGCs的数量，连续6周。Thy1-CFP小鼠体内成像结果与C57BL/6小鼠免疫组化染色结果相当。与单独注射微球相比，微球与HPM联合注射可引起更长、更高的眼压升高峰。初始IOP升高6周后，单独给药微珠组RGC损失率为25.0±1.3%，而给药微珠+ HPM组为33.2±1.9%。这些结果表明，注射微珠+ HPM是一种更有效的建立小鼠慢性IOP升高模型的方法。此外，该技术可用于体内成像，为监测RGC丢失的进展提供了一种有效且无创的方法。
The present study aimed to compare microbead injection with and without hydroxypropyl methylcellulose (HPM) in order to establish an experimental animal model of glaucoma. This model was established in C57BL/6 mice and transgenic mice expressing cyan fluorescent protein (CFP) under the control of the Thy1 promoter in retinal ganglion cells (RGCs). C57BL/6 mice aged between 12 and 20 weeks old were randomly separated into three groups, which received different injections into the anterior chamber of the eye. Group A (microbead) received 2 µl microbeads (10×106 beads/ml) and 1 µl air. Group B (microbeads + HPM) received 2 µl microbeads and 1 µl HPM. Group C (control group) received 2 µl PBS and 1 µl air. The intraocular pressure (IOP) was measured with a tonometer under topical anesthesia daily for 1 month. A single injection of microbeads, with or without HPM, induced consistent IOP elevation when compared with the control group. Thy1-CFP mice received an injection of 2 µl microbeads and 1 µl HPM into the anterior chamber of the eyes, and the number of CFP+ RGCs was subsequently assessed in vivo by confocal scanning laser microscopy in the same area of the retina weekly for 6 weeks. The results from in vivo imaging of Thy1-CFP mice were comparable with the immunohistochemical staining results from the C57BL/6 mice. The combined injection of microbeads and HPM induced longer and higher peaks of IOP elevation when compared with the microbeads alone. The rate of RGC loss following the administration of microbeads alone was 25.0±1.3% 6 weeks after the initial IOP elevation, while it was 33.2±1.9% following the administration of microbeads + HPM. These results indicate that the injection of microbeads + HPM is a more effective method of establishing a mouse model with chronic elevation of IOP. In addition, the in vivo imaging that can be used with this technique provides an effective and noninvasive approach for monitoring the progress of RGC loss.
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影响因子：
1.2
作者：
Feng, Liang;Chen, Hui;Liu, Xiaorong
通讯作者：
Liu, Xiaorong
影响因子：
2.5
作者：
García, M;Ruiz-Ederra, J;Vecino, E
通讯作者：
Vecino, E
影响因子：
1.7
作者：
Altintas, A. G. Kocak;Anayol, M. A.;Simsek, S.
通讯作者：
Simsek, S.
影响因子：
3.4
作者：
Morrison, JC;Moore, CG;Johnson, EC
通讯作者：
Johnson, EC
影响因子：
3.7
作者：
Lorenzetto E;Ettorre M;Pontelli V;Bolomini-Vittori M;Bolognin S;Zorzan S;Laudanna C;Buffelli M
通讯作者：
Buffelli M