Microtubule interactions with the cell cortex causing nuclear movements in Saccharomyces cerevisiae.
Microtubule interactions with the cell cortex causing nuclear movements in Saccharomyces cerevisiae.
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DOI：
10.1083/jcb.149.4.863
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发表时间：
2000-05-15
期刊：
影响因子：
--
通讯作者：
Cooper JA
中科院分区：
文献类型：
--
作者：
Adames NR;Cooper JA
在芽殖酵母的有丝分裂过程中，细胞核首先移动到母芽颈，然后进入芽颈。这两种运动都依赖于细胞质微管与皮质的相互作用。我们研究了活细胞中这些运动的机制，使用视频分析GFP标记的微管在野生型细胞和EB 1和Arp 1突变体，这是有缺陷的第一和第二步，分别。我们发现，核运动的颈部主要是介导的微管末端的捕获在一个皮层区域的初期芽网站或芽尖，然后微管解聚。微管与捕获位点的有效相互作用需要微管足够长和动态以探测皮层。相反，纺锤体向颈部的运动是由微管沿着芽皮层沿着介导的，这需要动力蛋白和动力蛋白。游离微管也可以沿着蕾和母体的皮层滑动。微管末端的捕获/收缩也有助于细胞核移动到颈部，并且可以在微管滑动受损时作为将细胞核移动到颈部的备用机制。相反，微管滑动可以将细胞核移动到颈部，即使捕获/收缩受损。
During mitosis in budding yeast the nucleus first moves to the mother-bud neck and then into the neck. Both movements depend on interactions of cytoplasmic microtubules with the cortex. We investigated the mechanism of these movements in living cells using video analysis of GFP-labeled microtubules in wild-type cells and in EB1 and Arp1 mutants, which are defective in the first and second steps, respectively. We found that nuclear movement to the neck is largely mediated by the capture of microtubule ends at one cortical region at the incipient bud site or bud tip, followed by microtubule depolymerization. Efficient microtubule interactions with the capture site require that microtubules be sufficiently long and dynamic to probe the cortex. In contrast, spindle movement into the neck is mediated by microtubule sliding along the bud cortex, which requires dynein and dynactin. Free microtubules can also slide along the cortex of both bud and mother. Capture/shrinkage of microtubule ends also contributes to nuclear movement into the neck and can serve as a backup mechanism to move the nucleus into the neck when microtubule sliding is impaired. Conversely, microtubule sliding can move the nucleus into the neck even when capture/shrinkage is impaired.
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影响因子：
7.8
作者：
Shaw, SL;Yeh, E;Maddox, P;Salmon, ED;Bloom, K
通讯作者：
Bloom, K
DOI：
10.1083/jcb.138.3.629
发表时间：
1997-08-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carminati JL;Stearns T
通讯作者：
Stearns T
影响因子：
3.3
作者：
Schwartz, K;Richards, K;Botstein, D
通讯作者：
Botstein, D
影响因子：
64.5
作者：
MUHUA, L;KARPOVA, TS;COOPER, JA
通讯作者：
COOPER, JA
酵母中皮质肌动蛋白斑块的运动。
影响因子：
7.8
作者：
Waddle, J A;Karpova, T S;Waterston, R H;Cooper, J A
通讯作者：
Cooper, J A