Regulation of growth of cultured hepatic epithelial cells by transferrin.
Regulation of growth of cultured hepatic epithelial cells by transferrin.
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转铁蛋白对培养的肝上皮细胞生长的调节。
DOI：
10.1016/0014-4827(87)90250-3
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发表时间：
1987
影响因子：
3.7
通讯作者：
Grisham,JW
中科院分区：
文献类型：
--
作者：
Tsao,MS;Sanders,GH;Grisham,JW
非致瘤性和贴壁依赖性肝上皮细胞系（WB-F344）的晚期传代细胞组成性产生胰岛素样生长因子II和转化生长因子β，在仅添加转铁蛋白的无血清培养基中生长。在转铁蛋白的存在下，表皮生长因子进一步增加人口增长，虽然表皮生长因子单独没有效果。胰岛素、血小板衍生生长因子和几种无机铁盐在没有转铁蛋白的情况下也不能有效地支持细胞生长;此外，这些因子不会增强转铁蛋白的作用。转铁蛋白的群体生长促进作用在0.5 nM或更高的浓度下发生，并且在约6 nM的浓度下达到最大作用。亲脂性铁螯合剂吡哆醛异烟酰腙铁（FePIH）可以完全模拟转铁蛋白对WB-F344细胞增殖的作用，但促进细胞增殖所需的FePIH的摩尔浓度比转铁蛋白的摩尔浓度高1000倍。这些结果表明，转铁蛋白在WB-F344细胞增殖中的关键功能可能是将铁输送到细胞中。无转铁蛋白时，WB-F344细胞的增殖停滞在G 0 G1期，加入转铁蛋白后，细胞进入S期并开始DNA合成前，需要一段时间的蛋白质合成。转铁蛋白的替代导致静止的WB-F344细胞不同步地循环。表皮生长因子不改变S期前的潜伏期的长度，但似乎刺激随后的[3 H]胸苷的摄取。转铁蛋白可以作为一个“能力”和/或“进展”的因素，允许这些上皮细胞在体外复制。
Late-passage cells of a nontumorigenic and anchorage-dependent hepatic epithelial line (WB-F344), which produce insulinlike growth factor II and transforming growth factor β constitutively, grow in serum-free medium supplemented only with transferrin. In the presence of transferrin, epidermal growth factor further augments population growth, although epidermal growth factor alone is without effect. Insulin, platelet-derived growth factor, and several inorganic iron salts are also ineffective in supporting cell growth in the absence of transferrin; furthermore, these factors do not augment the action of transferrin. The population growth-promoting effect of transferrin occurs at concentrations of 0.5 nM or greater and the maximal effect is reached with a concentration of approximately 6 nM. A lipophilic iron chelator, ferric pyridoxal isonicotinoyl hydrazone (FePIH), can fully mimic the effect of transferrin on the proliferation of WB-F344 cells, but the molar concentration of FePIH required to promote cell proliferation is 1000-fold higher than the molar concentration of transferrin. These results suggest that the critical function of transferrin in the proliferation of WB-F344 cells may be in the delivery of iron to the cells. In the absence of transferrin the proliferation of WB-F344 cells is arrested in serum-free medium in the G 0 G 1 phase, and a period of protein synthesis after the addition of transferrin is necessary before the cells can proceed to S phase and initiate DNA synthesis. Replacement of transferrin causes quiescent WB-F344 cells to cycle parasynchronously. Epidermal growth factor does not alter the length of the latency period prior to S phase but appears to stimulate the uptake of [3 H] thymidine subsequently. Transferrin may act as a “competence” and/or “progression” factor, allowing the replication of these epithelial cells in vitro.
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铁在人类白血病细胞系生长中的作用
影响因子：
5.6
作者：
M. Titeux;U. Testa;F. Louache;P. Thomopoulos;H. Rochant;J. Breton‐Gorius
通讯作者：
J. Breton‐Gorius
在无血清培养物中铁促进转化细胞的生长。
影响因子：
4.7
作者：
Paul Basset;J. Zwiller;M. Revel;Guy Vincendon
通讯作者：
Guy Vincendon
通过暴露于 N-甲基-N-硝基-N-亚硝基胍，在表达“椭圆形”细胞表型的培养的大鼠肝上皮细胞中诱导表型和核型变化。
DOI：
--
发表时间：
1985
期刊：
The American journal of pathology
影响因子：
--
作者：
Tsao,MS;Grisham,JW;Nelson,KG;Smith,JD
通讯作者：
Smith,JD
DOI：
10.1172/jci111768
发表时间：
1985-03
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
R. Taetle;K. Rhyner;J. Castagnola;D. To;J. Mendelsohn
通讯作者：
R. Taetle;K. Rhyner;J. Castagnola;D. To;J. Mendelsohn
大鼠支持细胞分泌的睾丸转铁蛋白的纯化和表征。
DOI：
10.1042/bj2180313
发表时间：
1984
期刊：
The Biochemical journal
影响因子：
--
作者：
Skinner,MK;Cosand,WL;Griswold,MD
通讯作者：
Griswold,MD