Plasma Unesterified Fatty-Acid Profile Is Dramatically and Acutely Changed under Ischemic Stroke in the Mouse Model.
Plasma Unesterified Fatty-Acid Profile Is Dramatically and Acutely Changed under Ischemic Stroke in the Mouse Model.
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作者：
Golovko SA;Golovko MY
虽然血浆生物标志物有助于快速准确地诊断缺血性卒中，以便立即治疗，但迄今为止尚未开发出此类生物标志物。在本研究中，我们验证了我们的假设，即血浆游离脂肪酸（FFA）在急性缺血性卒中的早期阶段发生改变。从永久性大脑中动脉闭塞（pMCAo）发作后2小时的小鼠以及假手术和对照动物收集血浆。2小时后，pMCAo显著改变了血浆FFA谱，极长n-3和n-6 FFA（包括20：4 n-6、22：4 n-6、22：5 n-6和22：6 n-3）的相对增加最显著，为2至3倍。血浆FFA谱的变化与缺血性损伤下脑磷脂水解释放FFA一致。这些结果首次确定了血浆FFA谱作为溶栓治疗窗内早期缺血性卒中的潜在生物标志物。需要进一步的研究来证实其在临床环境中的特异性和敏感性。
Although plasma biomarkers would facilitate rapid and accurate diagnosis of ischemic stroke for immediate treatment, no such biomarkers have been developed to date. In the present study, we tested our hypothesis that plasma free fatty acids (FFA) are altered at early stages of acute ischemic stroke. Plasma was collected from mice 2 h after permanent middle cerebral artery occlusion (pMCAo) onset, as well as from sham-operated and control animals. After 2 hours, pMCAo significantly changed the plasma FFA profile with the most dramatic 2-to 3-fold relative increase in very long n-3 and n-6 FFA including 20:4n-6, 22:4n-6, 22:5n-6, and 22:6n-3. Changes in plasma FFA profile are consistent with FFA liberation from brain phospholipid hydrolyzed under ischemic insult. These results identify, for the first time, the plasma FFA profile as a potential biomarker for an early ischemic stroke within the therapeutic window for thrombolytic treatment. Further studies are required to confirm its specificity and sensitivity in clinical settings.
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影响因子：
1.9
作者：
Brose SA;Golovko SA;Golovko MY
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