Chronic shifts in the length and phase of the light cycle increase intermittent alcohol drinking in C57BL/6J mice.
Chronic shifts in the length and phase of the light cycle increase intermittent alcohol drinking in C57BL/6J mice.
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DOI：
10.3389/fnbeh.2015.00009
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发表时间：
2015
影响因子：
3
通讯作者：
Gulick D
中科院分区：
文献类型：
--
作者：
Gamsby JJ;Gulick D
简介：轮班工人，例如，卫生保健专业人员、卡车司机和工厂工人被迫维持与他们的自然昼夜节律不一致的每日周期，因此需要频繁地重新调整这些周期。这种轮班工作引起的昼夜节律紊乱（CD）与睡眠障碍增加和酗酒有关。尽管如此，已经证明很难在小鼠模型中模拟CD诱导的酒精消耗变化，这是确定CD增加酒精摄入的机制的重要一步。这项研究检查了光周期的频繁变化是否会增加容易饮酒的小鼠系的自由获取酒精摄入量。研究方法：测量雄性C57 BL/6 J小鼠的自由获取酒精摄入量、水摄入量和轮跑活动模式，同时将小鼠维持在正常的12 HR光周期上以获得基线数据2周。然后将小鼠暴露于12 h和18 h的交替光周期，在18 HR光周期期间光开始提前8 h。光周期每3天旋转一次，共21天。结果如下：相位提前和延迟的重复模式，以及光周期长度的同时变化，将小鼠转变为间歇性饮酒的模式，而不改变饮水量。轮跑活动表明，小鼠在CD期间无法重置其行为时钟，表现出恒定的低水平活动，在黑暗阶段开始时没有活动峰值，而在晨光阶段活动更大。结论：有可能使用相移和光周期变化模式来模拟CD对C57 BL/6 J小鼠酒精摄入量的影响。使用这个模型，我们证明了小鼠在CD期间开始间歇性饮酒，并且这种酒精摄入量的增加与总体活动或总体液体摄入量的增加无关。
Introduction: Shift workers—e.g., health care professionals, truck drivers, and factory workers—are forced to maintain daily cycles at odds with their natural circadian rhythms and as a consequence need to frequently readjust these cycles. This shift work-induced circadian desynchrony (CD) is associated with increased sleep disorders and with alcohol abuse. Nonetheless, it has proven difficult to model CD-induced changes in alcohol consumption in mouse models, which is an important step toward identifying the mechanisms by which CD increases alcohol intake. This study examined whether frequent changes in the light cycle could increase free access alcohol intake in a mouse line that readily consumes alcohol. Methods: Free access alcohol intake, water intake, and wheel-running activity patterns of male C57BL/6J mice were measured while the mice were maintained on a normal 12HR photoperiod for baseline data for 2 weeks. The mice were then exposed to an alternating photoperiod of 12 h and 18 h, with light onset advanced 8 h during the 18HR photoperiod. The photoperiods rotated every 3 days, for 21 days total. Results: The repeated pattern of phase advances and delays, with a concurrent change in the length of the photoperiod, shifted mice to a pattern of intermittent alcohol drinking without altering water intake. Wheel running activity demonstrated that mice were unable to reset their behavioral clocks during CD, showing constant, low-level activity with no peak in activity at the start of the dark phase and greater activity during the morning light phase. Conclusion: It is possible to model CD effects on alcohol intake in C57BL/6J mice using a pattern of phase shifts and changes in the photoperiod. Using this model, we demonstrate that mice begin intermittent drinking during CD, and this increase in alcohol intake does not correlate with an increase in overall activity or in overall fluid intake.
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影响因子：
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