Physiological, biochemical, and molecular differences in chloroplast synthesis between leaf and corolla of cabbage (Brassica rapa L. var. chinensis) and rapeseed (Brassica napus L.)
Physiological, biochemical, and molecular differences in chloroplast synthesis between leaf and corolla of cabbage (Brassica rapa L. var. chinensis) and rapeseed (Brassica napus L.)
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卷心菜（Brassica rapa L. var. chinensis）和油菜（Brassica napus L.）叶和花冠叶绿体合成的生理、生化和分子差异
DOI：
10.1007/s10725-016-0241-4
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发表时间：
2016-12
影响因子：
4.2
通讯作者：
Hu Zongli
中科院分区：
文献类型：
--
作者：
Zhu Mingku;Meng Xiaoqing;Chen Guoping;Dong Tingting;Yu Xiaohui;Cai Jing;Hu Zongli
虽然近年来已经积累了大量关于叶绿体和有色体生物合成的信息，但是对于绿色组织和有色体组织之间的生理、生化和分子差异的细节仍然知之甚少。本研究分析了甘蓝和油菜叶片和花冠之间的色素积累、质体超微结构以及叶绿体合成相关基因的表达。结果表明，与萼片和叶片相比，两种花瓣的叶绿素含量和Chla/B比值均较低，但类胡萝卜素含量较高。因此，超微结构的观察表明，花瓣的质体发育被逮捕或抑制。此外，生化分析结果与mRNA水平的相关性表明，几乎所有的叶绿素生物合成基因（尤其是下游基因）和叶绿体早期发育和光合作用所需的质体基因在叶片中的转录量均高于花瓣。此外，不同的色素积累在花冠中也证实了与叶绿素积累和/或叶绿体发育相关的基因的表达的变化。总的来说，这些结果有助于更好地理解植物组织特异性叶绿体发育的分子机制。
Although much information regarding the chloroplast and chromoplast biosynthesis has been accumulated in recent years, details of the physiological, biochemical, and molecular differences between green tissues and colorful chromoplast tissues are still poorly understood. In this study, the pigment accumulation, plastid ultrastructure, and the expression of genes involved in chloroplast synthesis were analyzed between leaf and corolla in cabbage and rapeseed. The results showed that both petals contained less chlorophyll contents and a lower ratio of Chl a/b, but contained higher carotenoid contents compared with that of sepals and leaves. Accordingly, ultrastructural observations indicated that plastid development of petals was arrested or inhibited. In addition, data obtained from biochemical studies were correlated with those at the mRNA level, the transcripts of almost all the chlorophyll biosynthetic genes (especially the downstream genes) and plastid genes required for early chloroplast development and photosynthesis were higher in leaves than that in petals. Besides, the different pigment accumulation in corollas was also corroborated by the changes in the expression of selected genes associated with chlorophyll accumulation and/or chloroplast development. Collectively, the results contribute to better understand the molecular mechanisms underlying tissue-specific chloroplast development in plants.
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DOI：
10.1007/bf02464880
发表时间：
1989-06-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
HIRATSUKA, J;SHIMADA, H;SUGIURA, M
通讯作者：
SUGIURA, M
DOI：
10.1016/j.bbabio.2014.11.011
发表时间：
2015-09
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
J. Rochaix;S. Ramundo
通讯作者：
J. Rochaix;S. Ramundo
影响因子：
1.6
作者：
Kusumi, Kensuke;Chono, Yoko;Iba, Koh
通讯作者：
Iba, Koh
DOI：
--
发表时间：
2009
期刊：
--
影响因子：
--
作者：
B. Salopek-Sondi;V. Magnus
通讯作者：
B. Salopek-Sondi;V. Magnus
影响因子：
11.4
作者：
Hajdukiewicz, PTJ;Allison, LA;Maliga, P
通讯作者：
Maliga, P