Genotypic diversity and unrecognized antifungal resistance among populations of Candida glabrata from positive blood cultures.
Genotypic diversity and unrecognized antifungal resistance among populations of Candida glabrata from positive blood cultures.
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DOI：
10.1038/s41467-023-41509-x
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发表时间：
2023-09-22
影响因子：
16.6
通讯作者：
Nguyen, M. Hong
中科院分区：
文献类型：
--
作者：
Badrane, Hassan;Cheng, Shaoji;Dupont, Christopher L.;Hao, Binghua;Driscoll, Eileen;Morder, Kristin;Liu, Guojun;Newbrough, Anthony;Fleres, Giuseppe;Kaul, Drishti;Espinoza, Josh L.;Clancy, Cornelius J.;Nguyen, M. Hong
长期以来的模型是，大多数血液感染（BSI）是由单一生物体引起的。我们对10例光滑念珠菌BSI患者的血培养（BC）瓶中的5 - 10株菌株进行全基因组测序。我们表明，BC包含混合种群的克隆，但遗传多样性菌株。来自两名患者的遗传上不同的菌株表现出在BSI期间可能重要的表型，包括对抗真菌剂和吞噬作用的敏感性差异。在这两名患者中，临床微生物学实验室恢复了氟康唑敏感指数菌株，但我们确定了混合的氟康唑敏感和耐药人群。药物敏感性的多样性可能具有临床相关性，因为临床实验室随后在持续性或复发性感染期间回收了氟康唑耐药菌株。在一名患者中，未被识别的呼吸缺陷型小菌落变异体对氟康唑耐药，并在小鼠念珠菌病期间毒力显著减弱。我们的数据表明，C.在BSI期间glabrata基因型和表型多样性。光滑念珠菌血流感染被认为主要由单一菌株引起。在这里，作者描述了10个个体血培养中光滑念珠菌的基因型和表型多样性，并发现患者体内药物敏感性和毒力的多样性。
The longstanding model is that most bloodstream infections (BSIs) are caused by a single organism. We perform whole genome sequencing of five-to-ten strains from blood culture (BC) bottles in each of ten patients with Candida glabrata BSI. We demonstrate that BCs contain mixed populations of clonal but genetically diverse strains. Genetically distinct strains from two patients exhibit phenotypes that are potentially important during BSIs, including differences in susceptibility to antifungal agents and phagocytosis. In both patients, the clinical microbiology lab recovered a fluconazole-susceptible index strain, but we identify mixed fluconazole-susceptible and –resistant populations. Diversity in drug susceptibility is likely clinically relevant, as fluconazole-resistant strains were subsequently recovered by the clinical laboratory during persistent or relapsing infections. In one patient, unrecognized respiration-deficient small colony variants are fluconazole-resistant and significantly attenuated for virulence during murine candidiasis. Our data suggest a population-based model of C. glabrata genotypic and phenotypic diversity during BSIs. Candida glabrata bloodstream infections are thought to be mostly caused by a single strain. Here, the authors describe genotypic and phenotypic diversity of Candida glabrata in ten individual blood cultures, and find diversity in drug susceptibility and virulence within patients.
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影响因子：
3.2
作者：
Gabaldón T;Carreté L
通讯作者：
Carreté L
机会性病原体念珠菌中基因组变异的模式表明，交配的存在和与人类的二次关联。
DOI：
10.1016/j.cub.2017.11.027
发表时间：
2018-01-08
期刊：
Current biology : CB
影响因子：
--
作者：
Carreté L;Ksiezopolska E;Pegueroles C;Gómez-Molero E;Saus E;Iraola-Guzmán S;Loska D;Bader O;Fairhead C;Gabaldón T
通讯作者：
Gabaldón T
影响因子：
3.2
作者：
Jacobsen MD;Duncan AD;Bain J;Johnson EM;Naglik JR;Shaw DJ;Gow NA;Odds FC
通讯作者：
Odds FC
影响因子：
3.2
作者：
Ahmad KM;Kokošar J;Guo X;Gu Z;Ishchuk OP;Piškur J
通讯作者：
Piškur J
影响因子：
4.8
作者：
Hallstrom, TC;Moye-Rowley, WS
通讯作者：
Moye-Rowley, WS